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Squid glycoproteins with structural similarities to Thy-1 and Ly-6 antigens

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Abstract

In an attempt to identify invertebrate homologs of Thy-1 antigen, the optic and central nervous tissue of squid was solubilized in deoxycholate and fractionated by lentil lectin affinity chromatography and gel filtration to yield small abundant glycoproteins. Material with biochemical similarities to Thy-1 was found and shown to consist of two glycoproteins that were ultimately purified using monoclonal antibody affinity columns. Both glycoproteins were sequenced to yield sequences of 84 residues for Sgp-1 and 92 residues for Sgp-2. The sequences were analyzed for similarities to Thy-1 and other Ig-related sequences, and Sgp-1 showed some similarities that were > 3 standard deviation units away from mean random scores when tested with the ALIGN program. However, the sequence patterns were not typical of Ig-related domains and the relationship of Sgp-1 to the Ig superfamily remains problematical. Sgp-2 showed no relationship to the Ig superfamily, but similarities to Ly-6 antigen sequences were noted that are in accord with an evolutionary relationship. The similarities included ten Cys residues in each sequence of which eight were matched in the best alignment given by the ALIGN program. Chemical evidence was obtained for glycophospholipid tails at the COOH-termini of Sgp-1 and Sgp-2 as is the case for Thy-1 and Ly-6 antigens.

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Abbreviations

GPL:

glycophospholipid

mAb:

monoclonal antibody

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Author notes

  1. Albert G.-D. Tsel

    Present address: FMD Division, Wellcome Biotechnology Limited, Ash Road, Pirbright, Woking, GU24 ONQ, Surrey, UK

  2. Jean Gagnon

    Present address: National Research Council Canada, Biotechnology Research Institute, A/S Hôpital Royal Victoria, Pavillon Hersey, 687 Avenue des Pins, Ouest, H3A 1A1, Montréal, Québec, Canada

Authors and Affiliations

  1. MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, OX1 3RE, Oxford, UK

    Alan F. Williams & Albert G.-D. Tsel

  2. MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, OX1 3QU, Oxford, UK

    Jean Gagnon

Authors
  1. Alan F. Williams
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  2. Albert G.-D. Tsel
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  3. Jean Gagnon
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Williams, A.F., Tsel, A.GD. & Gagnon, J. Squid glycoproteins with structural similarities to Thy-1 and Ly-6 antigens. Immunogenetics 27, 265–272 (1988). https://doi.org/10.1007/BF00376121

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