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Squid Giant Axons Synthesize NF Proteins

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Abstract

Squid giant axon has been an excellent model system for studying fundamental topics in neurobiology such as neuronal signaling. It has been also useful in addressing the questions of local protein synthesis in the axons. Incubation of isolated squid giant axons with [35S]methionine followed by immunoprecipitation with a rabbit antibody against all squid neurofilament (NF) proteins demonstrates the local synthesis of a major 180 kDa NF protein and of several NF proteins of lower molecular weights. Their identification as NF proteins is based on their absence in the preimmune precipitates. Immunoprecipitates washed with more stringent buffers confirmed these results. Our data are at variance with a recent study based on the same experimental procedure that failed to visualize the local synthesis of NF proteins by the giant axon and thereby suggested their exclusive derivation from nerve cell bodies (as reported by Gainer et al. in Cell Mol Neurobiol 37:475–486, 2017). By reviewing the pertinent literature, we confute the claims that mRNA translation is absent in mature axons because of a putative translation block and that most proteins of mature axons are synthesized in the surrounding glial cells. Given the intrinsic axonal capacity to synthesize proteins, we stress the glial derivation of axonal and presynaptic RNAs and the related proposal that these neuronal domains are endowed with largely independent gene expression systems (as reported by Giuditta et al. in Physiol Rev 88:515–555, 2008).

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Acknowledgements

We thank the staff of the Marine Biological Laboratory (Woods Hole, MA, USA) for providing squid and scientific facilities. We acknowledge financial aid by grant 910242 from NATO, and grants from MURST, the University of Naples, PS “Mezzogiorno” and “Ricerca di Ateneo 2016” from the University of Naples Federico II.

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Authors and Affiliations

  1. Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Naples, Italy

    Marianna Crispino & Antonio Giuditta

  2. Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale 1, Napoli, 80121, Italy

    Jong Tai Chun

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  1. Marianna Crispino
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  2. Jong Tai Chun
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  3. Antonio Giuditta
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Correspondence to Marianna Crispino.

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Crispino, M., Chun, J.T. & Giuditta, A. Squid Giant Axons Synthesize NF Proteins. Mol Neurobiol 55, 3079–3084 (2018). https://doi.org/10.1007/s12035-017-0561-z

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