Abstract
A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene. The cells grew well in the alpha MEM medium supplemented with 5% dialyzed fetal calf serum (dFCS), but not with less than 1% dFCS. Therefore we examined various medium components and obtained an improved medium which supported cell growth at low serum concentrations. The production of hGH by the cells was also enhanced in this medium.
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Abbreviations
- CHO:
-
Chinese hamster ovary
- hGH:
-
human growth hormone
- dFCS:
-
dialyzed fetal calf serum
- dhfr:
-
dihydroforate reductase
- MTX:
-
methotrexate
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Hata, Ji., Tamura, T., Yokoshima, S. et al. Chemically defined medium for the production of biologically active substances of CHO cells. Cytotechnology 10, 9–14 (1992). https://doi.org/10.1007/BF00376095
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DOI: https://doi.org/10.1007/BF00376095