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Role of cytokines in controlling connective tissue gene expression

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Abstract

Recently, the role of cytokines in controlling gene expression of connective tissue components has been increasingly emphasized. Many cytokines have been shown to have specific effects on gene expression of connective tissue components, and the roles of cytokines in controlling connective tissue metabolism during wound healing and in fibrosis have increasingly been discussed. In this article, the effects of cytokines on regulation of gene expression of connective tissue components, especially of type I collagen were described. We analysed transcriptional control of the α1(I) collagen gene by TNF-α by means of DNA mediated transfection experiments using recombinant plasmids in which the promoter region of the human α1(I) collagen had been fused to the chloramphenicol acetyl-transferase (CAT) gene, in human dermal fibroblasts. It was found that TNF-α reduced α1(I) collagen transcription through at least up to −107 bp upstream of the human α1(I) collagen promoter gene in dermal fibroblasts.

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  1. Department of Dermatology, Kawasaki Medical School, 577 Matsushima, 701-01, Kurashiki, Japan

    A. Hatamochi, K. Mori & H. Ueki

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  2. K. Mori
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Hatamochi, A., Mori, K. & Ueki, H. Role of cytokines in controlling connective tissue gene expression. Arch Dermatol Res 287, 115–121 (1994). https://doi.org/10.1007/BF00370729

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