Abstract
The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100–500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation and specific antibody productivity were independent of the centrifugal effect, and gave the same results as in the case of no exposure to centrifugal force. High density cultivation of the mouse-human hybridoma X87 line was obtained by a perfusion system where the cells were separated from the culture medium by continuous centrifugation. In the serum-free culture, the maximum viable cell density exceeded 107 cells/ml, and monoclonal antibody was stably produced for 37 days. The results in this culture were equivalent to those obtained by intermittent centrifugal cell separation from the culture medium, and separation by gravitational settlement.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hamamoto K, Ishimaru K and Tokashiki M (1989) Perfusion culture of hybridoma cells using a centrifuge to separate cells from culture mixture. Journal of Fermentation and Bioengineering 67: 190–194.
Himmelfarb P, Thayen PS and Martin HE (1969) Spin filter culture: the propagation of mammalian cells in suspension. Science 164: 555–557.
Knazek PA, Gullino PM, Kohler PO and Dedrick RI (1972) Cell culture on artificial capillaries. Science 178: 65–67.
Lydersen K, Pugh G, Paris S, Sharma P and Noll A (1985) Ceramic matrix for large scale cell culture. Bio/Technology 3: 63–37.
Murakami H, Shimomura T, Nakamura T, Ohashi H, Shinohara K and Omura H (1984) Development of a basal medium for serum-free cultivation of hybridoma cells in high density. J. Agric. Chem. Soc. Japan 58: 575–583.
Murakami H, Shimomura T, Ohashi H, Hashizume S, Tokashiki M, Shinohara K, Yasumoto K, Nomoto K and Omura H (1985) Growth and differentiation of cells in defined environment: Serum free stirred culture of human-human hybridoma lines, pp. 111–116. Kodansha Ltd. and Springer-Verlag.
Posillio EG (1986) Microcapsulation technology for largescale antibody production. Bio/Technology 4: 114–117.
Sato S, Kawamura K, Hanai N and Fujiyoshi N (1985) Growth and differentiation of cells in defined environment: Production of interferon and monoclonal antibody using a novel type of perfusion vessel, pp. 123–126. Kodansha Ltd. and Springer-Verlag.
Tokashiki M, Hamamoto K, Takazawa Y and Ichikawa Y (1988) High-density culture of mouse-human hybridoma cells using a new perfusion culture vessel. Kagakukoga-ku Ronbunshu 14: 337–341.
Tolbert W and Feder J (1983) Large-scale cell culture technology. Annual reports on fermentation processes 6: 35–100.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tokashiki, M., Arai, T., Hamamoto, K. et al. High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge. Cytotechnology 3, 239–244 (1990). https://doi.org/10.1007/BF00365487
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00365487