Abstract
Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.
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References
DankbergF and PersidskyMD (1976) A test of granulocyte membrane integrity and phagocyte function. Cryobiology 13: 430–432.
EdidinM (1970) A rapid quantitative fluorescence assay for cell damage by cytotoxic antibodies. J. Immunol. 104: 1303.
Goetghebeur S and Hu W-S Cultivation of anchoragedependent animal cells in microsphere-induced aggregate culture. Appl. Microbiol. Biotechnol. (accepted)
GrayDWR and MorrisPJ (1987) The use of fluorescein diacetate and ethidium bromide as a viability stain for isolated islets of Langerhans. Stain Technol. 62: 373–379.
HimesVB and HuWS (1987) Attachment and growth of mammalian cells on microcarriers with different ion exchange capacities. Biotechnol. Bioeng. 29: 1155–1163.
HuWS, GiardDJ, and WangDIC (1985) Serial propagation of mammalian cells on microcarriers. Biotechnol. Bioeng. 27: 1466–1476.
JohanssonA and NielsenV (1980) Biosilon: A new microcarrier. Develop. Biol. Stand. 46: 125.
JonesKH and SenftJA (1985) An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide. J. Histochem. Cytochem. 33: 77–79.
KeeseDR and GiaeverI (1983) Cell growth on liquid microcarriers. Science 219: 1448.
KuoMJ, LewisC, MartinRA, MillerRE, SchoenfeldRA, ScheckJM, and WildiBS (1981) Growth of anchoragedependent mammalian cells on glycine-derivatized polystyrene in suspension culture. In Vitro 17: 901.
LevineDW, WangDIC, and ThillyWG (1979) Optimization of growth surface parameters in microcarrier cell culture. Biotechnol. Bioeng. 21: 821–845.
NilssonK, BuzsakyF, and MosbachK (1986) Growth of anchorage-dependent cells on macroporous microcarriers. Bio/Technology 4: 989–990.
NorrgrenG, EbendalT, GebbC and WikstromH (1982) The use of Cytodex-3 microcarriers and reduced serum media for the production of nerve growth promoters from chicken heart cells. Develop. Biol. Stand. 46: 125.
ReiterM, HohenwarterO, GaidaT, ZachN, SchmatzC, BlumlG, WeiganF, NilssonK, and KatingerH (1990) The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidized bed reactors. Cytotechnology 3: 271–277.
ReuvenyS, MizragiA, KotlerM, and FreemanA (1983) Factors affecting cell attachment spreading and growth on derivatized microcarriers. II: Introduction of hydrophobic elements. Biotechnol. Bioeng. 25: 469.
VaraniJ, DameM, BealsTF, and WassJA (1983) Growth of three established cell lines on glass microcarriers. Biotechnol. Bioeng. 25: 1359.
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Nikolai, T.J., Peshwa, M.V., Goetghebeur, S. et al. Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining. Cytotechnology 5, 141–146 (1991). https://doi.org/10.1007/BF00365430
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DOI: https://doi.org/10.1007/BF00365430