Abstract
Mouse strain and tissue distribution analyses indicate that the new antiserum A anti-A-Tla b recognizes the cell-surface product governed by the previously serologically undetectable Qa-I b allele. This cell-surface product has therefore been called Qa-1.2. Three levels of anti-Qa-1.2 cytotoxicity in the presence of complement have been observed: high, intermediate, and zero lysis. In general, high levels of lysis correlate with the presence of the Qa-1 b allele, while zero levels of lysis correlate with the presence of the Qa-1 aallele. The A.CA strain reacts with both anti-Qa-1.1 and anti-Qa-1.2 and may possess a third allele, Qa-1 d. Several strains including B6-H-2 k react in an intermediate fashion. Recombinant strain analyses indicate that this intermediate reaction may be due to modifying genes within the H-2D region.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amos, D. B., Gorer, P. A., and Mikulska, Z. B.: An analysis of an antigenic system in the mouse (the H-2 system). Proc. R. Soc. Lond. (Biol.) 144: 369–380, 1955
Boyse, E. A., Old, L. J., and Chouroulinkov, I.: Cytotoxic test for demonstration of mouse antibody. Methods Med. Res. 10: 39–47, 1964
Boyse, E. A., Old, L. J., and Stockert, E.: The TL (thymus leukemia) antigen: A review. In P. Grabar and P. A. Miescher (eds.): Immunopathology IVth International Symposium, pp. 23–40, Schwabe and Co., Basel/Stuttgart, 1965
Boyse, E. A., Stockert, E., and Old, L. J.: Isoantigens of the H-2 and Tla loci of the mouse. Interactions affecting their representation on thymocytes. J. Exp. Med. 128: 85–95, 1968
Boyse, E. A., Hubbard, L., Stockert, E., and Lamm, M. E.: Improved complementation in the cytotoxic test. Transplantation 10: 446–449, 1970
Cantor, H., Hugenberger, J., McVay-Boudreau, L., Eardley, D. D., Kemp, J., Shen, F-W., and Gershon, R. K.: Immunoregulatory circuits among T-cell sets. Identification of a subpopulation of T-helper cells that induces feedback inhibition. J. Exp. Med. 148: 871–877, 1978
Cook, R. G., Vitteta, E. S., Uhr, J., and Capra, J. D.: Structural studies on the murine la alloantigens. V. Evidence that the structural gene for the I-E/C beta polypeptide is encoded within the I-A subregion. J. Exp. Med. 149: 981–986, 1979
Flaherty, L.: The Tla region of the mouse: identification of a new serologically defined locus, Qa-2. Immunogenetics 3: 533–539, 1976
Flaherty, L. and Zimmerman, D.: Surface mapping of mouse thymocytes. Proc. Natl. Acad. Sci. U.S.A. 75: 1990–1993, 1979
Flaherty, L. and Rinchik, E.: A new allele and antigen at the Tla locus. Immunogenetics 11: 205–208, 1980
Flaherty, L.: The Tla region antigens. In M. Dorf (ed.): The Role of the Major Histocompatibility Complex in Immunobiology, pp. 33–57, Garland Press, New York, 1981
Forman, J.: H-2 unrestricted cytotoxic T cell activity against antigens controlled by genes in the Qa/Tla region. J. Immunol. 123: 2451–2455, 1979
Gorer, P. A. and O'Gorman, P.: The cytotoxic activity of isoantibodies in mice. Transplant. Bull. 3: 142–143, 1956
Hämmerling, G., Hämmerling, U., and Flaherty, L.: Qat-4 and Qat-5 new antigens governed by the Tla region and identified by monoclonal antibodies. J. Exp. Med. 150: 108–116, 1979
Jones, P. P., Murphy, D. B., and McDevitt, H. O.: Two gene control of the expression of a murine la antigen. J. Exp. Med. 148: 925–939, 1978
Kastner, D. L. and Rich, R. R.: H-2 nonrestricted cytotoxic responses to an antigen encoded telmeric to H-2D. J. Immunol. 122: 196–201, 1979
Kastner, D. L., Rich, R. R., and Shen, F-W: Qa-l-associated antigens. I. Generation of H-2-nonrestricted cytotoxic T lymphocytes specific for determinants of the Qa-1 region. J. Immunol. 123: 1232–1238, 1979
Klein, J. and Chiang, C. L.: A new locus (H-2T) at the D end of the H-2 complex. Immunogenetics 6: 235–243, 1978
Klein, J., Flaherty, L., VandeBerg, J. L., and Shreffler, D. C.: H-2 haplotypes, genes, regions, and antigens: First listing. Immunogenetics 6: 489–512, 1978
Lindahl, K. F.: Unrestricted killer cells recognize an antigen controlled by a gene linked to Tla. Immunogenetics 8: 71–76, 1979
Lindahl, K. F. and Hausmann, B.: Qed-1-a target for unrestricted killing by T cells. Eur. J. Immunol. 10: 289–298, 1980
Rothenberg, E. and Boyse, E. A.: Synthesis and processing of molecules bearing thymus leukemia antigen. J. Exp. Med. 150: 777–791, 1979
Scheid, M., Boyse, E. A., Carswell, E. A., and Old, L. J.: Serologically demonstrable alloantigens of mouse epidermal cells. J. Exp. Med. 135: 938–955, 1972
Shen, F-W., Boyse, E. A., and Cantor, H.: Preparation and use of Ly antisera. Immunogenetics 2: 591–595, 1975
Stanton, T. H. and Boyse, E. A.: A new serologically defined locus, Qa-1, in the Tla-region of the mouse. Immunogenetics 3: 525–531, 1976
Stanton, T. H., Calkins, C. E., Jandinski, J., Schendel, D. J., Stutman, O., Cantor, H., and Boyse, E. A.: The Qa-1 antigenic system. Relation of Qa-1 phenotypes to lymphocyte sets, mitogen responses, and immune functions. J. Exp. Med. 148: 963–973, 1978
Stanton, T. H.: Expression of Qa-1 on mitogen-stimulated cells. Immunogenetics 9: 597–600, 1979
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Flaherty, L., Rinchik, E. & DiBiase, K. New complexities at the Qa-1 locus. Immunogenetics 13, 339–346 (1981). https://doi.org/10.1007/BF00364499
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00364499