Abstract
The cytotoxic effector cells that recognize HLA-D-region determinants and their precursors were characterized using monoclonal antibodies against human T lymphocytes and T-cell subsets. These studies were performed using MLC combinations giving rise to cytotoxic cells specific for both class I (HLA-A, B, C) and class 11 (HLA-D-region) antigens, and then tested against target cells displaying relevant antigens of only one class. Both class I and class II specific CTL (cytotoxic T lymphocytes) were inhibited by treatment with the OKT3 monoclonal antibody and complement, indicating that the effector cells were T lymphocytes. A major portion.of class II specific CTL, and their precursors, were inhibited by OKT4 and complement, while class I specific CTL from the same cultures were not. The T4+T8 — cell subset has previously been associated with helper or inducer functions, but not with cytotoxicity. The present findings indicate that class I and class 11 specific CTL, and their precursors, are different on the basis of the class of target antigen recognized and on the basis of surface phenotype detected by monoclonal antibodies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albrechtsen, D., Arnesen, E., and Thorsby, E.: Cell-mediated cytotoxicity directed against HLA-D gene products. Transplantation 27: 338–341, 1979
Ball, E., Feighery, C., and Stastny, P.: Cell mediated cytotoxicity against HLA-D region products expressed in monocytes and B lymphocytes. 111. Inhibition by monoclonal antibodies and study of an HLA-B/DR recombinant. Hum. Immunol. 2: 305–313, 1981
Danilovs, J., Ayoub, G., and Terasaki, P.: B lymphocyte isolation by thrombin-nylon wool. In P. Terasaki (ed.): Histocompatibility Testing 1980, pp. 287–288, UCLA Tissue Typing Laboratory, Los Angeles, California, 1980
Feighery, C. and Stastny, P.: HLA-D region-associated determinants serve as targets for human cellmediated lysis. J. Exp. Med. 149: 485–494, 1979
Feighery, C. and Stastny, P.: Cell-mediated cytotoxicity against HLA-D region products expressed in monocytes and B lymphocytes. I. Blocking by unlabelled cells and inhibition by antisera. Immunogenetics 10: 31–38, 1980a
Feighery, C. and Stastny, P.: Cell-mediated cytotoxicity against HLA-D region products expressed in monocytes and B lymphocytes. 11. HLA-A- or B-antigen compatibility is not required for HLA-D region directed cytotoxicity. Immunogenetics 10: 39–43, 1980b
Ing, P., Falk, J., Letarte, M., Delovitch, T., and Falk, R.: Serologic cross reactions of murine and human la antigens. Transplant. Proc. 11: 1745–1747, 1979
Johnsen, H.: Human B-blast specific target determinants in CML: a panel study. Tissue Antigens 15: 199–209, 1980
Kisielow, P., Hirst, J., Shiku, H., Beverly, P., Hoffman, M., Boyse, E., and Oettgen, H.: Ly antigen markers for functionally distinct subsets of thymus-derived lymphocytes of the mouse. Nature 253: 219–220, 1975
Lampson, L. and Levy, R.: Two populations of la-like molecules on a human B cell line. J. Immunol. 125: 293–299, 1980
Moretta, L., Mingari, M., Sekaly, P., Moretta, A., Chapuis, B., and Cerottini, J.: Surface marker of cloned human T cells with various cytolytic activities. J. Exp. Med. 154: 569–574, 1981
Okada, M. and Henney, C.: The differentiation of cytotoxic T cells in vitro. Il. Amplifying factor(s) produced in primary mixed lymphocyte culture against K/D stimuli require the presence of Lyt2+ cells but not Lytl+ cells. J. Immunol. 125: 300–307, 1980
Okada, J. and Stastny, P.: Alloantigens expressed on a subset of unstimulated human T lymphocytes that generate suppressor function. Immunogenetics, (in press), 1982
Ortaldo, J., Sharrow, S., Timonen, T., and Herberman, R.: Determination of surface antigens on highly purified human NK cells by flow cytometry with monoclonal antibodies. J. Immunol. 127: 2401–2409, 1981
Reinherz, E., Kung, P., Goldstein, G., and Schlossman, S.: Separation of functional subsets of human T cells by a monoclonal antibody. Proc. Natl. Acad. Sci. U.S.A. 76: 4061–4065, 1979a
Reinherz, E., Kung, P., Goldstein, G., and Schlossman, S.: Further characterization of the human inducer T cell subset defined by monoclonal antibody. J. Immunol. 123: 2894–2896, 1979b
Reinherz, E., Kung, P., Goldstein, G., and Schlossman, S.: A monoclonal antibody reactive with the human cytotoxic/suppressor subset previously defined by a heteroantiserum termed TH2. J. Immunol. 124: 1301–1307, 1980
Staff, Research Resources Branch, NIAID. NIH lymphocyte microcytotoxicity technique. In J. Ray, D. Hare, P. Pedersen, and D. Mullally (eds.): Manual of Tissue Typing Techniques, pp. 22–24, DHEW Publication No. (NIH) 78-545, Washington D. C. Government Printing Office, 1976
Stastny, P.: Mixed lymphocyte cultures in rheumatoid arthritis. J. Clin. Invest. 57:1148–1157, 1976
Swain, S.: Association of Ly phenotypes, T cell functions and MHC recognition. Fed. Proc. 39:3111–3113, 1980
Swain, S. and Panfili, P.: Helper cells activated by allogeneic H-2K or H-2D differences have a Ly phenotype distinct from those responsive to I differences. J. Immunol. 122:383–391, 1979
Swain, S., Dennert, G., Wormsley, S., and Dutton, R.: The Lyt phenotype of a long-term allospecific T cell line. Both helper and killer activities to IA are mediated by Ly-1 cells. Eur. J. Immunol. 11:175–180, 1981
Vidovic, D., Juretic, A., Nagy, Z., and Klein, J.: Lyt phenotype of primary cytotoxic T cells generated across the A and E region of the H-2 complex. Eur. J. Immunol. 11:499–504, 1981
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ball, E.J., Stastny, P. Cell-mediated cytotoxicity against HLA-D-Region products expressed in monocytes and B lymphocytes. Immunogenetics 16, 157–169 (1982). https://doi.org/10.1007/BF00364402
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00364402