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The organization, localization and nucleotide sequence of the histone genes of the midge Chironomus thummi

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Abstract

Several histone gene repeating units containing the genes for histones H1, H2A, H2B, H3 and H4 were isolated by screening a genomic DNA library from the midge Chironomus thummi ssp. thummi. The nucleotide sequence of one complete histone gene repeating unit was determined. This repeating unit contains one copy of each of the five histone genes in the order and orientation 〈H3 H4〉 〈H2A H2B〉 H1〉. The overall length is 6262 bp. The orientation, nucleotide sequence and inferred amino acid sequence as well as the chromosomal arrangement and localization are different from those reported for Drosophila melanogaster. The codon usage also shows marked differences between Chironomus and Drosophila. Thus the histone gene structure reported for Drosophila is not typical of all insects.

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References

  • Benton WD, Davis RW (1977) Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196:180–182

    Google Scholar 

  • Chen EY, Seeburg PH (1985) Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170

    Google Scholar 

  • Doenecke D (1988) Histones, histone variants and postsynthetic histone modifications. In: Kahl G (ed) Architecture of eukaryotic genes. VCH, Weinheim, pp 123–141

    Google Scholar 

  • Domier LL, Rivard JJ, Sabatini LM, Blumenfeld M (1986) Drosophila virilis histone gene clusters lacking H1 coding segments. J Mol Evol 23:149–158

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    Google Scholar 

  • Frischauf A-M, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842

    Google Scholar 

  • Graceffa P, Wang LLA, Stafford WF (1988) Caldesmon. Molecular weight and subunit composition by analytical ultracentrifugation. J Biol Chem 263:14196–14202

    Google Scholar 

  • Hägele K (1970) DNS-Replikationsmuster der Speicheldrüsenchromosomen von Chironomiden. Chromosoma 31:91–138

    Google Scholar 

  • Hankeln T, Schmidt ER (1990) A new foldback transposable element TFB1 found in histone genes of the midge Chironomus thummi. J Mol Biol 215:477–482

    Google Scholar 

  • Hentschel CC, Birnstiel ML (1981) The organization and expression of histone gene families. Cell 25:301–313

    Google Scholar 

  • Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197

    Google Scholar 

  • Hoyer-Fender S, Grossbach U (1988) Histone H1 heterogeneity in the midge Chironomus thummi. Eur J Biochem 176:139–152

    Google Scholar 

  • Kirsh AL, Groudine M, Challoner PB (1989) Polyadenylation and U7 snRNP-mediated cleavage: alternative modes of RNA 3′ processing in two avian histone H1 genes. Genes Dev 3:2172–2179

    Google Scholar 

  • Kremer H, Hennig W (1990) Isolation and characterization of a Drosophila hydei histone DNA repeat unit. Nucleic Acids Res 18:1573–1580

    Google Scholar 

  • Langer-Safer PR, Levine M, Ward DC (1982) Immunological method for mapping genes on Drosophila polytene chromosomes. Proc Natl Acad Sci USA 79:4381–4385

    Google Scholar 

  • Lifton RP, Goldberg ML, Karp RW, Hogeness DS (1977) The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. Cold Spring Harbor Symp Quant Biol 42:1047–1051

    Google Scholar 

  • Mannironi C, Bonner WM, Hatch LL (1989) H2AX, a histone isoprotein with a conserved C-terminal sequence, is encoded by a novel mRNA with both DNA replication type and polyA 3′ processing signals. Nucleic Acids Res 17:9113–9126

    Google Scholar 

  • Matsuo Y, Yamazaki T (1989) tRNA derived insertion element in histone gene repeating unit of Drosophila melanogaster. Nucleic Acids Res 17:225–238

    Google Scholar 

  • Maxson R, Cohn R, Kedes L, Mohun T (1983) Expression and organization of histone genes. Annu Rev Genet 17:239–277

    Google Scholar 

  • Mohr E, Trieschmann L, Grossbach U (1989) Histone H1 in two subspecies of Chironomus thummi with different genome sizes: homologous chromosome sites differ largely in their content of a specific H1 variant. Proc Natl Acad Sci USA 86:9308–9312

    Google Scholar 

  • Murphy TJ, Blumenfeld M (1986) Nucleotide sequence of a Drosophila melanogaster H1 histone gene. Nucleic Acid Res 14:5563

    Google Scholar 

  • Pardue ML, Kedes LH, Weinberg ES, Birnstiel ML (1977) Localization of sequences coding for histone messenger RNA in the chromosomes of Drosophila melanogaster. Chromosoma 63:135–151

    Google Scholar 

  • Saigo K, Millstein L, Thomas Jr CA (1980) The organization of Drosophila melanogaster histone genes. Cold Spring Harbor Symp Quant Biol 45:815–827

    Google Scholar 

  • Sanicola M, Ward S, Childs G, Emmons SW (1990) Identification of a Caenorhabditis elegans histone H1 gene family. J Mol Biol 212:259–268

    Google Scholar 

  • Schmidt ER (1981) The development of a 120 pb repetitive DNA sequence in Chironomus thummi is correlated to the duplication of defined chromosomal segments. FEBS Lett 129:21–24

    Google Scholar 

  • Schmidt ER (1984) Clustered and interspersed repetitive DNA sequence family of Chironomus. J Mol Biol 178:1–15

    Google Scholar 

  • Schmidt ER, Vistorin G, Keyl H-G (1980) An AT-rich DNA component in the genomes of Chironomus thummi thummi and Chironomus thummi piger. Chromosoma 76:35–45

    Google Scholar 

  • Schmidt ER, Keyl H-G, Hankeln T (1988) In situ localization of two haemoglobin gene clusters in the chromosome of 13 species of Chironomus. Chromosoma 96:353–359

    Google Scholar 

  • Smith HO, Birnstiel ML (1976) Simple method for DNa restriction site mapping. Nucleic Acids Res 3:2387–2398

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–519

    Google Scholar 

  • Sturm RA, Dalton S, Wells JRE (1988) Conservation of histone H2A/2B intergene regions: a role for the H2B specific element in divergent transcription. Nucleic Acids Res 16:8571–8586

    Google Scholar 

  • van Daal A, White EM, Gorovsky MA, Elgin SCR (1988) Drosophila has a single copy of the gene encoding a highly conserved histone H2A variant of the H2A. F/Z type. Nucleic Acids Res 16:7487–8497

    Google Scholar 

  • Wells D, McBride C (1989) A comprehensive compilation and alignment of histones and histone genes. Nucleic Acids Res [Suppl] 17:311–346

    Google Scholar 

  • Wobus U (1975) Molecular characterization of an insect genome: Chironomus thummi. Eur J Biochem 59:287–293

    Google Scholar 

  • Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and puc 19 vectors. Gene 33:103–119

    Google Scholar 

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  1. Institut für Genetik, Johannes Gutenberg-Universität, Saarstrasse 21, W-6500, Mainz, Federal Republic of Germany

    Thomas Hankeln & Erwin R. Schmidt

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  1. Thomas Hankeln
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  2. Erwin R. Schmidt
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by H. Jäckle

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Hankeln, T., Schmidt, E.R. The organization, localization and nucleotide sequence of the histone genes of the midge Chironomus thummi . Chromosoma 101, 25–31 (1991). https://doi.org/10.1007/BF00360683

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  • DOI: https://doi.org/10.1007/BF00360683

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