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Purification and translation of ovalbumin, conalbumin, ovomucoid and lysozyme messenger RNA

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Abstract

Messenger RNAs for 4 egg white proteins (ovalbumin, conalbumin, ovomucoid and lysozyme) were assayed in a cell-free, protein-synthesizing system derived from rabbit reticulocytes. Each of these messengers was purified about 33-fold from hen oviduct polysomal RNA using oligo(dT) cellulose. The apparent minimal translational efficiencies varied from 22 translations for each ovalbumin mRNA to less than 1 for lysozyme mRNA. These messengers are not polycistronic with each other since they have distinct sedimentation values of: lysozyme, 8.5S; ovomucoid, 11S; ovalbumin, 15S; and conalbumin 18S. Several aspects of this system indicate that it will be valuable for dissecting the fine control of mRNA metabolism.

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References

  1. Palmiter, R. D., Catlin, G. H., and Cox, R. F., Cell Differentiation, in press.

  2. PalmiterR. D., J. Biol. Chem. 247, 6450 (1972).

    Google Scholar 

  3. PalmiterR. D. and GutmanG. A., J. Biol. Chem. 247, 6459 (1972).

    Google Scholar 

  4. PalmiterR. D., J. Biol. Chem. 247, 6770 (1972).

    Google Scholar 

  5. PalmiterR. D. and HainesM. E., J. Biol. Chem. 248, 2107 (1973).

    Google Scholar 

  6. PalaciosR., PalmiterR. D., and SchimkeR. T., J. Biol. Chem. 247, 2316 (1972).

    Google Scholar 

  7. Palmiter, R. D. and Smith, L. T., manuscript in preparation.

  8. PalmiterR. D., J. Biol. Chem. 248, 2095 (1973).

    Google Scholar 

  9. RhoadsR. E., McKnightG. S., and SchimkeR. T., J. Biol. Chem. 246, 7407 (1971).

    Google Scholar 

  10. SchutzG., BeatoM., and FeigelsonP., Biochem. Biophys. Res. Commun. 50, 486 (1972).

    Google Scholar 

  11. RosenfeldG. C., ComstockJ. P., MeansA. R., and O'MalleyB. W., Biochem. Biophys. Res. Commun. 46, 1695 (1972).

    Google Scholar 

  12. PalaciosR., SullivanD., SummersN. M., KielyM. L., and SchimkeR. T., J. Biol. Chem. 248, 540 (1973).

    Google Scholar 

  13. Haines, M. E., Carey, N. H., and Palmiter, R. D., J. Biol. Chem., manuscript submitted.

  14. Palmiter, R. D., manuscript in preparation.

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  1. Richard D. Palmiter

    Present address: The Biological Laboratories, Harvard University, 02138, Cambridge, Massachusetts, USA

Authors and Affiliations

  1. Dept. of Biochemistry, Searle Research Laboratories, High Wycombe, Bucks., England

    Richard D. Palmiter & Lynne T. Smith

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  1. Richard D. Palmiter
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  2. Lynne T. Smith
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Palmiter, R.D., Smith, L.T. Purification and translation of ovalbumin, conalbumin, ovomucoid and lysozyme messenger RNA. Molecular Biology Reports 1, 129–134 (1973). https://doi.org/10.1007/BF00357152

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  • DOI: https://doi.org/10.1007/BF00357152

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