Sensory transduction in an insect mechanoreceptor: Linear and nonlinear properties

Abstract

Mechanotransduction in the femoral tactile spine of the cockroach, Periplaneta americana, was examined as a function of displacement of the spine axially in its socket. Linear behaviour was analyzed by measurement of the frequency response function between displacement and action potential output using sinusoidal stimulation and random noise stimulation. The measured frequency response functions can be well fitted by a relationship which is a fractional power of complex frequency. This power was close to 0.5 for all experiments. To distinguish between the effects of nonlinearity and of inherent variability, the averaged responses of the preparation to repeated sequences of pseudorandom noise were compared to those from experiments in which continuous pseudorandom noise were used. The lack of sensitivity of the coherence function to these two methods of measurement suggests that mechanical stimuli are encoded into action potentials with a large signal-to-noise ratio. The low value of the coherence function which is characteristics of insect mechanoreceptors is therefore due to the strong non-linearity of their responses. To investigate the nonlinear properties of transduction, the second-order frequency response function of the tactile spine was measured for random noise stimulation experiments. Two models of the transduction process were considered in which a linear element with memory was cascaded with a nonlinear element without memory in the two possible configurations. Comparison of the experimental second-order frequency response functions with predictions based upon these two models and the measured first-order frequency response suggests that the transduction mechanism can be modelled by a linear element, which may be associated with the viscoelastic properties of the dendritic tubular body, and a zeromemory nonlinearity, which is most likely to be rectification by the dendritic membrane.