Abstract
The spatial distribution of chlorophyll in three coral species carrying invertebrate symbionts was determined using spectral imaging techniques. From each pixel of the image, full fluorescence spectral data was obtained as well as comparative ratios of fluorescence. The multipixel fluorescence map and the relative-intensity fluorescence ratios demonstrated a high concentration of chlorophyll a next to the pits of Cryptochirus coralliodytes in Favites halicora. Spectral similarity maps of Goniastrea retiformis infested with Lithophaga lessepsiana and of Millepora dichotoma infested with Savignium milleporum revealed relatively higher chlorophyll concentrations in these two corals next to the symbionts. We hypothesize that the invertebrate symbionts fertilize their immediate surroundings with their excreta, enhancing algal growth. The spectral analysis system used in this study made it possible to distinguish such changes by spatial quantitation of the fluorescence emitted from small surface areas.
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References
Achituv Y, Mizrahi L (1996) Recycling of ammonium within a hydrocoral (Millepora dichotoma)-zooxanthellae-cirripede Savignium milleporum symbiotic association. Bull mar Sci 58:856–860
Bell RJ (1972) Introductory Fourier transform spectroscopy. Academic Press, London
Bythell JC (1988) A total nitrogen and carbon budget for the elkhorn coral Acropora palmata (Lamarck). In: Choat JH et al. (eds) Proc 6th int Coral Reef Symp. Symposium Executive Committee, Townsville, pp 535–540
Chamberlin J (1978) The principles of interferometric spectroscopy. Wiley and Sons, New York
Cook CB, D'Elia CF (1987) Are natural populations of zooxanthellae ever nutrient-limited? Symbiosis 4:199–212
Cook PA, Stewart BA, Achituv Y (1991) The symbiotic relationship between the hydrocoral Millepora dichotoma and the barnacle Savignium milleporum. Hydrobiologia 216/217:285–290
Garini Y, Katzir N, Cabib D, Buckwald RA, Soenksen D, Malik Z (1996) Fluorescence spectral imaging techniques and its applications. In: Wang XF, Herman B (eds) Fluorescence imaging spectroscopy and microscopy. Wiley and Sons, New York, pp 87–124
Geze M, Morliere P, Maziere JC, Smith KM, Santus R (1993) Lysosomes, a key target of hydrophobic photosensitizers proposed for photochemotherapeutic applications. J Photochem Photobiol (Ser B) 20:23–35
Hadfield MG (1976) Molluscs associated with living tropical corals. Micronesica 12:133–148
Hardy JT, Hoge FE, Yungel JK, Dodge RE (1992) Remote detection of coral “bleaching” using pulsed-laser fluorescence spectroscopy. Mar Ecol Prog Ser 88:247–255
Hoegh-Guldberg O, Smith GJ (1989) Influence of the population density of zooxanthelae and supply of ammonium on the biomass and characteristics of the reef corals Seriatopora hystrix and Stylophora pistillata. Mar Ecol Prog Ser 57:173–186
Johannes RE, Coles SL, Kuenzel NT (1970) The role of zooplankton in the nutrition of some scleractinian corals. Limnol Oceanogr 15:579–586
Johannes RE, Wiebe WJ (1970) Method for determination of coral tissue biomasa and composition. Limnol Oceanogr 15:822–824
Kirk JTO (1983) Light and photosynthesis in aquatic ecosystems. Cambridge University Press, Cambridge
Kleemann KH (1980) Boring bivalves and their host corals from the Great Barrier Reef. J mollusc Stud 46:13–54
Lewis DH, Smith DC (1971) The autotrophic nutrition of symbiotic marine coelenterates with special reference to hermatypic corals. I. Movement of photosynthetic products between the symbionts. Proc R Soc (Ser B) 178:111–129
Malik Z, Cabib D, Buckwald RA, Talmi A, Garini Y, Lipson SG (1996) Fourier transform multipixel spectroscopy and spectral imaging for quantitative cytology. J Microscopy 182:133–140
Mazel CH (1995) Spectral measurements of fluorescence emission in Caribbean enidarians. Mar Ecol Prog Ser 120:185–191
Meyer JL, Schultz ET (1985) Tissue condition and growth rate of corals associated with schooling fish. Limnol Oceanogr 30:157–166
Muller-Parker G, McCloskey LR, Hoegh-Guldberg O, McAuley PJ (1994) Effect of ammonium enrichment on animal and algal biomass of the coral Pocillopora damicornis. Pacif Sci 48:273–283
Muscatine L, D'Elia CF (1978) The uptake, retention, and release of ammonium by reef corals. Limnol Oceanogr 23:725–734
Muscatine L, Falkowski PG, Dubinsky Z, Cook PA, McCloskey LR (1989) The effect of external nutrient resources on the population dynamics of zooxanthellae in a reef coral. Proc R Soc (Ser B) 236:311–324
Muscatine L, McCloskey LR, Marian RE (1981) Estimating the daily contribution of carbon from zooxanthellae to coral animal respiration. Limnol Oceanogr 26:601–611
Potts FA (1915) Hapalocaricinus, the gall-forming crab, with some notes on the related genus Cryptochirus. Pap Dep mar Biol Carnagie Instn Wash 8:33–69
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Communicated by O. Kinne, Oldendorf/Luhe
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Simon-Blecher, N., Achituv, Y. & Malik, Z. Effect of epibionts on the microdistribution of chlorophyll in corals and its detection by fluorescence spectral imaging. Marine Biology 126, 757–763 (1996). https://doi.org/10.1007/BF00351342
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DOI: https://doi.org/10.1007/BF00351342