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Primary biliary cirrhosis sera recognize not only gp210 but also proteins of the p62 complex bearing N-acetylglucosamine residues from rat liver nuclear envelope

Anti-p62 complex antibody in PBC

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Abstract

We have recently observed reactivity of primary biliary cirrhosis (PBC) sera with several proteins bearing N-acetylglucosamine residues from rat liver nuclear envelopes. The aim of this study was to characterize the reactive antigens. Sera from 31 patients with PBC, 30 with rheumatoid arthritis (RA) and 30 with Sjögren's syndrome (SS) were examined. Rim-like immunofluorescence staining was observed in 15 of 31 (48%) sera from patients with PBC, in 1 of 30 with RA and in 1 of 30 with SS. Upon immunoblotting using preparations of whole rat liver nuclear envelopes and their Triton X 100-KCl extract as antigen souces, a 200 kDa protein band was observed in 9 of sera with PBC. Furthermore, upon immunoblotting using the wheat germ aggulutinin-bound fraction of rat liver envelope as antigen, 62, 60 and 54 kDa protein bands corresponding to components of the p62 complex in the nuclear pore complex (Kita et al. Biochem. 113, 377–382) were observed in 7, 5 and 6 samples respectively, of the 31 PBC sera. Our data suggest that PBC sera recognize not only the 210 kDa protein but also the p62 complex proteins.

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Abbreviations

ANA:

antinuclear antibody

AMA:

anti-mitochondrial antibodies

IF:

immunofluorescence

LAP2:

lamina-associated polypeptide 2

LBR:

lamin B receptor

anti-NBP 60:

anti-nuclear localization signal binding protein 60

NE:

nuclear envelope

NPC:

nuclear pore complex

PBC:

primary biliary cirrhosis

RA:

rheumatoid arthritis

SLE:

systemic lupus erythematosus

SS:

Sjögren's syndrome

WGA:

wheat germ agglutinin

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Authors and Affiliations

  1. Keigu Medical Clinic, 2-2 Ichbanishinaka-cho, Tsurumi-ku, 230, Yokohama, Kanagawa-ken, Japan

    K. Miyachi

  2. Health Sciences Research Institute, 2-2 Ichbanishinaka-cho, Tsurumi-ku, 230, Yokohama, Kanagawa-ken, Japan

    K. Miyachi

  3. Tokyo Metropolitan Ebara Hospital, Tokyo, 4-5-10 Higashiyukigaya Outa-ku, Tokyo, Japan

    M. Shibata

  4. Kawasaki Chuou Hospital, Kawasaki, 2-9-1 Tamachi Kawasaki-ku, Kawasaki, Japan

    Y. Onozuka

  5. Faculty of Science, Niigata University, 950-21, Niigata, Japan

    T. Horigome

  6. Department of Biochemistry, Faculty of Science, Niigata University, 2-Igarashi, 950-21, Niigata, Japan

    T. Horigome

Authors
  1. K. Miyachi
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  2. M. Shibata
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  3. Y. Onozuka
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  4. F. Kikuchi
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  5. N. Imai
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  6. T. Horigome
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Miyachi, K., Shibata, M., Onozuka, Y. et al. Primary biliary cirrhosis sera recognize not only gp210 but also proteins of the p62 complex bearing N-acetylglucosamine residues from rat liver nuclear envelope. Molecular Biology Reports 23, 227–234 (1996). https://doi.org/10.1007/BF00351173

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