Skip to main content
SpringerLink
Log in

Construction of a porcine YAC library and mapping of the cardiac muscle ryanodine receptor gene to Chromosome 14q22–q23

  • Original Contributions
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

Large-scale physical mapping of the porcine genome has been limited because up to now no suitable genomic libraries for this purpose have been available. Therefore, we have constructed a yeast artificial chromosome (YAC) library from porcine lymphocytes. The library was cloned in the amplifiable vector pCGS966. A total of 10080 YAC clones was obtained and has been ordered into 105 96-well microtiter plates. An average insert size of 300 kb was calculated from the analysis of 78 randomly selected clones, giving a onefold coverage of the porcine genome. To analyze the complexity, we have screened the library for five different genes and isolated four different clones containing parts of three of these genes. One YAC clone harboring parts of the porcine cardiac muscle ryanodine receptor (RYR2) gene allowed us to assign this locus to Chromosome (Chr) 14q22-q23. The data were confirmed by PCR analysis of a rodent-porcine hybrid cell panel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Albertsen, H.M., Abderrahim, H., Cann, H.M., Dausset, J., Le, P.D., Cohen, D. (1990). Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents. Proc. Natl. Acad. Sci. USA 87, 4256–4260.

    Google Scholar 

  • Anand, R., Riley, J.H., Butler, R., Smith, J.C., Markham, A.F. (1990). A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage. Nucleic Acids Res. 18, 1951–1956.

    Google Scholar 

  • Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (1990). Current Protocols in Molecular Biology. (New York: John Wiley and Sons).

    Google Scholar 

  • Barlow, D., Bates, G., Lehrach, H., Poustka, A. (1989). Course manual of EMBO Practical Course.

  • Brenig, B., Brem, G. (1990). Genomic organization and analysis of the 5′ end of the porcine ryanodine receptor gene (ryrl). FEBS Lett. 298, 277–279.

    Google Scholar 

  • Brownstein, B.H., Silverman, G.A., Little, R.D., Burke, D.T., Korsmeyer, S.J., Schlessinger, D., Olson, M.V. (1989). Isolation of single-copy human genes from a library of yeast artificial chromosome clones. Science 244, 1348–1351.

    Google Scholar 

  • Burgers, P.M., Percival, K.J. (1987). Transformation of yeast spheroplasts without cell fusion. Anal. Biochem. 163, 391–397.

    Google Scholar 

  • Burke, D.T., Carle, G.F., Olson, M.V. (1987). Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236, 806–812.

    Google Scholar 

  • Chevalet, C., Corpet, F. (1986). Statistical decision rules concerning synteny or independence between markers. Cell Genet. 43, 132–139.

    Google Scholar 

  • Coulson, A., Waterston, R., Kiff, J., Sulston, J., Kohara, Y. (1988). Genome linking with yeast artificial chromosomes. Nature 335, 184–186

    Google Scholar 

  • Dolf, G., Stranzinger, G. (1986). Pig gene mapping. Assignment of the genes for mannose phosphate isomerase (MPI) and nucleoside phosphorylase (NP) to chromosome no. 7. Genet. Sel. Evol. 18, 375–384.

    Google Scholar 

  • Garza, D., Ajioka, J.W., Burke, D.T., Hartl, D.L. (1989). Mapping the Drosophila genome with yeast artificial chromosomes. Science 246, 641–646.

    Google Scholar 

  • Green, E.D., Olson, M.V. (1990). Systematic screening of yeast artificialchromosome libraries by use of the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87, 1213–1217.

    Google Scholar 

  • Gustavsson, G. (1988). Standardized GTG karyotype of the domestic pig. Hereditas 109, 151–177.

    Google Scholar 

  • Haley, C.S., Archibald, A., Andersson, L., Bosma, A.A., Davies, W., Fredholm, M., Geldermann, H., Groenen, M., Gustavsson, I., Ollivier, L., Tucker, E.M., Van de Weghe, A. (1990). The pig gene mapping project-PiGMaP. Proc. 4th WCGALP XIII, pp. 67–70.

  • Hillier, L., Green, P. (1991). OSP: a computer program for choosing PCR and DNA sequencing primers. PCR Methods Appl. 1, 124–128.

    Google Scholar 

  • Larin, Z., Monaco, A.P., Lehrach, H. (1991). Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc. Natl. Acad. Sci. USA 88, 4123–4127.

    Google Scholar 

  • Leeb, T., Schmölzl, S., Brem, G., Brenig, B. (1993). Genomic organization of the porcine skeletal muscle ryanodine receptor (RYR1) gene coding region 4624–7929. Genomics 18, 349–354.

    Google Scholar 

  • Libert, F., Lefort, A., Okimoto, R., Womack, J., Georges, M. (1993). Construction of a bovine genomic library of large yeast artificial chromosome clones. Genomics 18, 270–276.

    Google Scholar 

  • MacLennan, D.H., Phillips, M.S. (1992). Malignant hyperthermia. Science 256, 789–794.

    Google Scholar 

  • Mattei, M.G., Giannini, G., Moscatelli, F., Sorrentino, V. (1994). Chromosomal localization of murine ryanodine receptor genes RYR1, RYR2, and RYR3 by in situ hybridization. Genomics 22, 202–204.

    Google Scholar 

  • McCormick, M.K., Shero, J.H., Cheung, M.C., Kan, Y.W., Hieter, P.A., Antonarakis, S.E. (1989). Construction of human chromosome 21-specific yeast artificial chromosomes. Proc. Natl. Acad. USA 86, 9991–9995.

    Google Scholar 

  • Mellink, C., Lahbib-Mansais, Y., Yerle, M., Gellin, J. (1993). Localization of four new markers to pig chromosomes 1, 6 and 14 by in situ hybridization. Cell Genet. 64, 256–260.

    Google Scholar 

  • Moir, D.T., Dorman, T.E., Smyth, A.P., Smith, D.R. (1993). A human genome YAC library in a selectable high-copy-number vector. Gene 125, 229–232.

    Google Scholar 

  • Müller, M., Brem, G. (1990). Nucleotide sequence of porcine insulin-like growth factor I: 5′ untranslated region, exons 1 and 2 and mRNA. Nucleic Acids Res. 18, 364.

    Google Scholar 

  • Nelson, D.L., Brownstein, B.H. (1994). YAC Libraries—A User's Guide. (New York: W.H. Freeman and Company).

    Google Scholar 

  • Otsu, K., Fujii, J., Periasamy, M., Difilippantonio, M., Uppender, M., Ward, D.C., MacLennan, D.H. (1993). Chromosome mapping of five human cardiac and skeletal muscle sarcoplasmic reticulum protein genes. Genomics 17, 507–509.

    Google Scholar 

  • Pachnis, V., Pevny, L., Costantini, F. (1990). Transfer of a yeast artificial chromosome carrying human DNA from Saccharomyces cerevisiae into mammalian cells. Proc. Natl. Acad. Sci. USA 87, 5109–5113.

    Google Scholar 

  • Qin, S., Zhang, J., Isaacs, C.M., Nagafuchi, S., JaniSait, S.N., Abel, K.J., Higgins, M.J., Nowak, N.J., Shows, T.B. (1993). A chromosome 11 YAC library. Genomics 16, 580–585.

    Google Scholar 

  • Rettenberger, G., Fries, R., Engel, W., Scheit, K.H., Dolf, G., and Hameister, H. (1994a). Establishment of a partially informative porcine somatic cell hybrid panel and assignment of the loci for transition protein 2 (TNP2) and protamine 1 (PRM1) to chromosome 3 and polyubiquitin (UBC) to chromosome 14. Genomics 21, 558–566.

    Google Scholar 

  • Rettenberger, G., Fredholm, M., Fries, R. (1994b). Chromosomal assignment of porcine microsatellites by use of a somatic cell hybrid mapping panel. Anim. Genet. (in press)

  • Rossi, J.M., Burke, D.T., Leung, J.C., Koos, D.S., Chen, H., Tilghman, S.M. (1992). Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts. Proc. Natl. Acad. Sci. USA 89, 2456–2460.

    Google Scholar 

  • Schedl, A., Larin, Z., Montoliu, L., Thies, E., Kelsey, G., Lehrach, H., Schutz, G. (1993). A method for the generation of YAC transgenic mice by pronuclear microinjection. Nucleic Acids Res. 21, 4783–4787.

    Google Scholar 

  • Schlessinger, D., Little, R.D., Freije, D., Abidi, F., Zucchi, I., Porta, G., Pilia, G., Nagaraja, R., Johnson, S.K., Yoon, J.-Y., Srivastava, A., Kere, J., Palmieri, G., Ciccodicola, A., Montanaro, V., Romano, G., Casamassimi, A., D'Urso, M. (1991). Yeast artificial chromosome-based genome mapping: some lessons from Xq24-q28. Genomics 11, 783–793.

    Google Scholar 

  • Sleister, H.M., Mills, K.A., Blackwell, S.E., Killary, A.M., Murray, J.C., Malone, R.E. (1992). Construction of a human chromosome 4 YAC pool and analysis of artificial chromosome stability. Nucleic Acids Res. 20, 3419–3425.

    Google Scholar 

  • Smith, D.R., Smyth, A.P., Moir, D.T. (1990). Amplification of large artificial chromosomes. Proc. Natl. Acad. Sci. USA 87, 8242–8246.

    Google Scholar 

  • Traver, C.N., Klapholz, S., Hyman, R.W., Davis, R.W. (1989). Rapid screening of a human genomic library in yeast artificial chromosomes for single-copy sequences. Proc. Natl. Acad. Sci. USA 86, 5898–5902.

    Google Scholar 

  • Zehetner, G., Lehrach, H. (1994). The reference library system—sharing biological material and experimental data. Nature 367, 489–491.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biochemistry, University of Munich, 81375, Munich, Germany

    T. Leeb

  2. Department of Clinical Genetics, University of Ulm, 89069, Ulm, Germany

    G. Rettenberger & H. Hameister

  3. Institute of Animal Breeding and Genetics, University of Veterinary Medicine, 1030, Vienna, Austria

    G. Brem

  4. Institute of Veterinary Medicine, University of Göttingen, Groner Landstrasse 2, 37073, Göttingen, Germany

    T. Leeb & B. Brenig

Authors
  1. T. Leeb
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Rettenberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Hameister
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Brem
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Brenig
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Leeb, T., Rettenberger, G., Hameister, H. et al. Construction of a porcine YAC library and mapping of the cardiac muscle ryanodine receptor gene to Chromosome 14q22–q23. Mammalian Genome 6, 37–41 (1995). https://doi.org/10.1007/BF00350891

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00350891

Keywords

Search

Navigation