Abstract
Attachment strength and colonization patterns of one barnacle (Balanus c.f. variegatus) and one polychaete species (Pomatoleios kraussii) on seven artificial substrata with surface tensions between 19 and 64.5 mNm-1 were studied between June 1991 and January 1992 at Laem Than (Chonburi province, Gulf of Thailand). The purpose of the present study was to demonstrate the role that surface tension plays under natural conditions in colonization success by these species. Does stronger adhesion to one substratum result in higher densities of specimens on that particular surface? Although both species adhere much better on substrata with higher surface tension than on surfaces with low surface tension (B. c.f. variegatus: between 0.04×105 and 16.35×105 Nm-2 on surfaces of 22 and 33.5 mNm-1, respectively; P. kraussii: between 0.22×105 and 1.73×105 Nm-2 on 19 and 33.5 mNm-1, respectively), colonization pattern is not influenced by surface tension. The major factors influencing settlement patterns seem to be space competition for B. c.f. variegatus and attraction to already settled adults by P. kraussii. The results show that the importance of surface tension in marine fouling control is limited because organisms colonize substrata successfully despite lower adhesion. Attachment strength is not a limiting factor under natural conditions.
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Absolom, D. R., van Oss, C. J., Genco, R. J., Francis, D. W., Zingg, W., Neumann, A. W. (1980). Surface thermodynamics of normal and pathological human granulocytes. Cell Biophys. 2: 113–126
Baier, R. E. (1973). Influence of the initial surface condition of materials on bioadhesion. In: Acker, R. F., Brown, B. F., DePalma, J. R., Iverson, W. P. Proc. 3rd int. congr. mar. corr. foul., Northwestern University Press, Evanston, p. 633–639
Becka, A., Loeb, G. (1984). Ease of removal of barnacles from various polymeric materials. Biotechnol Bioengng 26: 1245–1251
Becker, K., Wahl, M. (1991). Influence of substratum surface tension on biofouling of artificial substrata in Kiel Bay (Western Baltic): in situ studies. Biofouling 4: 275–291
Burchard, R. P., Rittschof, D., Bonaventura, J. (1990). Adhesion and motility of gliding bacteria on substrata with different surface free energies. Appl. envirl Microbiol 56(8): 2529–2534
Busscher, H. J. (1985). Surface free energies and the adhesion of oral bacteria. Ph. D. Thesis, Riksuniversitet te Groningen, Groningen
Cook, P. A., Henschel, J. R. (1984). The importance of a primary film of microorganisms on the subsequent establishment of a macrofouling community. In: Proc. 6th int. congr. mar. corr. Foul., Athens - Greece, p. 211–220
Characklis, W. G., Cooksey, K. E. (1983). Biofilms and microbial fouling. Adv. appl. Microbiol. 29: 93–138
Crisp, D. J. (1984). Overview of research on marine invertebrate larvae, 1940–1980. In: Costlow, J. D., Tipper, R. C. (eds.) Marine corrosion and biodeterioration — an interdisciplinary study. E. & F. N. Spon. Ltd., London, p. 103–126
Crisp, D. J., Ryland, J. S. (1960). Influence of filming and of surface texture on the settlement of marine organisms. Nature, Lond. 185: 119
Crisp, D. J., Walker, G., Young, G. A., Yule, A. B. (1985). Adhesion and substrate choice in mussels and barnacles. J. Colloid interface Sci. 104(1): 40–50
Dayton, P. K. (1971). Competition, disturbance and community organization: the provision and subsequent utilization of space in a rocky intertidal community. Ecol. Monogr. 41: 351–389
Dexter, S. C. (1979). Influence of substratum critical surface tension on bacterial adhesion—in situ studies. J. Coll. Interf. Sci. 70(2): 346–354
Dillon, P. S., Maki, J. S., Mitchell, R. (1989). Adhesion of Enteromorpha swarmenrs to microbial films. Microb. Ecol. 17: 39–47
Eiben, R. (1976). Der Einfluss der Benetzungsspannung und Ionen auf die Substratbesiedlung und das Einsetzen der Metamorphose bei Bryozoenlarven (Bowerbankia gracilis). Mar. Biol. 37: 249–254
Fletcher, M., Loeb, G. I. (1979). Influence of substratum characteristics on the attachment of a marine Pseudomonad to solid surfaces. Appl. envirl Microbiol. 37(1): 67–72
Fletcher, R. L., Baier, R. E. (1984). Influence of surface energy on the development of the green alga Enteromorpha. Mar. Biol. Lett. 5: 251–254
Fromageot, H. P. M., Groves, J. N., Sears, A. R., Brown, J. F. (1976). The interaction of macromolecular solutions with macromolecular monolayers adsorbed on hydrophobic surfaces. J. Biomed. Res. 10: 455–469
Gaines, S., Brown, S., Roughgarden, J. (1985). Spatial variation in larval concentrations as a cause of spatial variation in settlement for the barnacle, Balanus glandula. Oeceologia 67: 267–272
Goupil, D. W., DePalma, V. A., Baier, R. E. (1980) Physical chemical characteristics of the macromolecular conditioning film in biological fouling. 5th int. Congr mar. Corr. Foul. p. 401–410
Gubbay, S. (1983). Compressive and adhessive strength of a variety of British barnacles. J. mar. biol. Ass. U.K. 63: 541–555
Hudon, C., Bourget, E. (1983). An integrated study of factors influencing the choice of the settling site of Balanus crenatus cyprid larvae. Can. J. Fish. aquat. Sciences 40: 1186–1194
Jackson, S. M., Jones, E. B. G. (1991). Interactions within biofilms: ruption of biofilm structure by protozoa. Kieler Meersforsch. (Sonderh. 8): 264–268
Karande, A. A., Udhayakumar M. (1989). Shell structure and shell strength in Cirripedes. Proc. Indian Acad. Sci. (anim. Sci.). 98(4): 223–231
Kirchman, D., Graham, S., Reish, D., Mitchell, R. (1982). Bacteria induce settlement and metamorphosis of Janua (Dexiospira) brasiliensis Grube (Polychaeta: Spirorbidea). J. exp. mar. biol. Ecol. 56: 153–163
Knight-Jones, E. W., Crisp, D. J. (1953). Gregariousness in barnacles in relation to the fouling of ships and to antifouling research. Nature, Lond. 171: 1–15
MacRitchie, F. (1972). The adsorption of proteins at the solid/liquid interface. J. Colloid interface Sci. 38(2): 484–488
Maki, J. S., Rittschof, D., Costlow, J. D., Mitchell, R. (1988). Inhibition of attachment of larval barnacles, Balanus amphitrite, by bacterial surface films. Mar. Biol. 97: 199–206
Maki, J. S., Rittschof, D., Mitchell, R. (1992). Inhibition of barnacle attachment to bacterial films: an investigation of physical properties. Microb. Ecol. 23: 97–106
Maki, J. S., Rittschof, D., Samuelsson, M.-O., Szewyk, U., Yule, A. B., Kjelleberg, S., Costlow, J. D., Mitchell, R. (1990). Effect of marine bacteria and their exopolymers on the attachment of barnacle cyprid larvae. Bull. mar. Sci. 46(2): 499–511
Maki, J. S., Rittschof, D., Schmidt, A. R., Snyder, A. G., Mitchell, R. (1989). Factors controlling attachment of bryozoan larvae: a comparison of bacterial films and unfilmed surfaces. Biol. Bull. mar. biol. Lab., Woods Hole 177: 295–302
Maroudas, N. G. (1975). Adhesion and spreading of cells on charged surfaces. J. theor. Biol. 49: 417–424
Mihm, J. W., Banta, W. C., Loeb, G. (1981). Effects of adsorbed organic and primary fouling films on bryozoan settlement. J. exp. mar. Biol. 54: 167–179
Neumann, A. W., Absolom, D. R., Francis, D. W., van Oss, C. J. (1980). Conversion tables of contact angles to surface tensions. Sep. purif. methods 9(1): 69–163
Rittschof, D., Branscomb, E. S., Costlow, J. D. (1984). Settlement and behavior to flow and surface in larval barnacles, Balamus amphitrite Darwin. J. exp. mar. Biol. Ecol. 82: 131–146
Rittschof, D., Costlow, J. D. (1989). Bryozoan and barnacle settlement in relation to initial surface wetability: a comparison of laboratory and field studies. In: Ros, E. D. (ed.) Topics in marine biology, Vol. 53. Sci. Mar., New York, p. 411–416
Robb, I. D. (1984). Stereo-biochemistry and function of polymers. In: Marshall, K. C. (ed.) Microbial adhesion and aggregation. Dahlem Konferenzen. Springer, Verlag, Berlin, p. 39–49
Roberts, D., Rittschof D., Holm, E., Schmidt, A. R. (1991). Factors influencing initial larval settlement: temporal, spatial and surface molecular components. J. exp. mar. Biol. Ecol. 150: 203–211
Scheltema, R. S., Williams, I. P., Shaw, M. A., Loudon, C. (1981). Gregarious settlement by the larvae of Hydroides dianthus (Polychaeta: Serpulidae). Mar. Ecol. Prog. Ser. 5: 69–74
Wahl, M. (1989). Marine epibiosis. I. Fouling and antifouling: some basic aspects. Mar. Ecol. Prog. Ser. 58: 175–189
Wu, S. (1973). Polar and nonpolar interactions in adhesion. J. Adhesion 5: 39–55
Yule, A. B., Crisp, D. J. (1983). Adhesion of cyprids of the larvae of the barnacle, Balanus balanoides, to clean and Athropodin treated surfaces. J. mar. biol. Ass. U.K. 63: 261–271
Yule, A. B., Walker, G. (1985). Settlement of Balanus balanoides: the effect of cyprid antennular secretion. J. mar. biol. Ass. U.K. 65: 707–712
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Communicated by O. Kinne, Oldendorf/Luhe
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Becker, K. Attachment strength and colonization patterns of two macrofouling species on substrata with different surface tension (in situ studies). Marine Biology 117, 301–309 (1993). https://doi.org/10.1007/BF00345675
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DOI: https://doi.org/10.1007/BF00345675