Summary
Cytological and histological observations have been made on the pancreatic islets of a diabetic (KK) strain of the mouse, and of control mice which belong to either C57BL/6 or ddN strains, and the following results were obtained.
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1.
In the pancreas of the diabetic mouse strain a certain number of irregularly contoured islets and occasional insular hypertrophy and hyperplasia are observed in contrast to the insular tissues of control animals.
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2.
The insular B cells of KK animals are seen to undergo cellular hypertrophy. The intracellular granules of this cell type are smaller in amount, colored darker and more coarse in size in KK individuals than in control animals.
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3.
In some insular tissues of KK mice there occur frequently varying degrees of sinusoidal dilatation of blood capillaries.
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4.
The cytoplasm of the insular A cells in KK animals is of a fine granulation which tends to be colored in a significantly less intense rose pink shade than that in control individuals.
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5.
The average proportion of the insulare A cells in KK mice is assumed to be strikingly small.
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6.
The dithizone reaction of the cytoplasm of the insular B cells in KK animals denotes the scarcity of the cellular zinc content.
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7.
The present morphological data on the pancreatic islets of the diabetic mouse strain have been discussed in relation to growth hormone secretion of the anterior pituitary of the animals.
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References
Bargmann, W.: Die Langerhansschen Inseln des Pankreas. In: Handbuch der mikroskopischen Anatomie des Menschen, Bd. VI/2. Berlin: Springer 1939.
Barrnett, R. J., R. B. Marshall, and A. M. Seligman: Histochemical demonstration of insulin in the islet of Langerhans. Endocrinology 57, 419–438 (1955).
Bornstein, J., E. Reid, and F. G. Young: The hyperglycemic action of blood from animals treated with growth hormone. Nature (Lond.) 168, 903–905 (1951).
Ferner, H.: Beiträge zur Histobiologie der Langerhansschen Inseln des Menschen mit besonderer Berücksichtigung der Silberzellen und ihrer Beziehung zum Pankreasdiabetes. Virchows Arch. path. Anat. 309, 87–136 (1942).
—: Das Inselsystem des Pankreas. Stuttgart: Georg Thieme 1952.
Gomori, G.: Observations with differential stains on human islet of Langerhans. Amer. J. Path. 17, 395–406 (1941).
Hagedorn, H. C., u. B. N. Jensen: Zur Mikrobestimmung des Blutzuckers mittels Ferricyanid. Biochem. Z. 135, 46–58 (1923).
Hultquist, G. T.: On the occurence of s. c. Silvercells in islet tumors and in the pancreas in cases of islet tumors. Gastroenterologia (Basel) 71, 193 (1946). Cit. from Ferner 1952.
Kondo, K., K. Nozawa, T. Tomita, and K. Ezaki: Inbred strains resulting from Japanese mice. Bull. exp. Anim. 6, 107–112 (1957). [In Japanese].
Lazarus, S. S., and B. Volk: The Pancreas in human and experimental diabetes. New York and London: Grune & Stratton 1962.
Maeda, T., T. Fujiwara, and K. Hironari: Histochemical demonstration of zinc. Bull. Kobe Med. College 5, 97–99 (1954) [In Japanese].
Maske, H.: Über die konkurrierende Komplexbildung zwischen Zinkinsulin und Substanzen aus dem intermediären Glukosestoffwechsel. Ber. ges. Physiol. 172, 163–164 (1955).
Nakamura, M.: A diabetic strain of the mouse. Proc. Jap. Acad. 38, 348–352 (1962).
—, and K. Yamada jr.: A further study of the diabetic (KK) strain of the mouse. F1 and F2 offspring of the cross between KK and C57BL/6 mice. Proc. Jap. Acad. 39, 489–493 (1963).
Park, C. R., D. H. Brown, M. Cornblath, W. H. Daughaday, and M. E. Krahl: The effect of growth hormone on glucose uptake by the isolated rat diaphragm. J. biol. Chem. 197, 151–166 (1952).
Park, C. R., D. H. Brown, M. Cornblath, M. E. Krahl, and W. H. Daughaday: Effect of growth hormone on the glucose uptake and glycogen synthesis by the rat diaphragm. Fed. Proc. 9, 212–213 (1950).
Terbrüggen, A.: Untersuchungen über Inselapparat und Inseladenome, insbesondere über die Zelltypen bei Diabetes mellitus und Spontanhypoglykämie. Virchows Arch. path. Anat. 315, 407–460 (1948).
Thiemer, K.: Zellbild der Langerhansschen Inseln beim Alloxandiabetes der hypophysektomierten Ratte und nach Zufuhr von Somatotropin bei normalen Ratten. Endokrinologie 30, 176–184 (1953).
Volk, B. W., M. G. Goldner, and H. Frank-Crowley: The effect of prolonged growth hormone administration on the pancreatic alpha cells in normal and hypophysectomized rats. Use of a modification of Davenport's silver impregnation method. Metabolism 4, 491–502 (1955).
Wrenshall, G. A., S. B. Andrus, and J. Mayer: High levels of pancreatic insulin coexistent with hyperglyoemia and degranulation of beta cells in mice with the hereditary obese-hyperglycemic syndrome. Endocrinology 56, 335–340 (1954).
Yamada, K.: (Unpublished data) (1964).
Yamada jr., K.: Chemical cytology of the mouse parathyroid gland. (In press) (1964).
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Acknowledgements. The author is indebted to Prof. Dr. Kaztjmaro Yamada for the guidance throughout this work. Further, he wishes to thank Profs. Drs. Kyoji Kondo and Tsutomu Sugahara for the supply of the experimental and control mice. Dr. Kazuyori Yamada's collaboration in the course of this investigation is gratefully acknowledged.
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Nakamura, M. Cytological and histological studies on the pancreatic islets of a diabetic strain of the mouse. Zeitschrift für Zellforschung 65, 340–349 (1965). https://doi.org/10.1007/BF00345635
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DOI: https://doi.org/10.1007/BF00345635