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Interaction between MHC-encoded products and cloned T cells

II. Analyses of Physiological Requirements Indicate Two Different Pathways of Stimulation by Class I Alloantigens

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Abstract

The interaction between class I major histocompatibility complex (MHC) products and T cells was studied using H-2Kb-specific alloreactive T-cell lines and clones obtained by repeated in vitro stimulation with allogeneic cells. Induction of proliferation of these T cells appeared to involve two signals: the H-2Kb alloantigen and interleukins. Immunopurified liposome-inserted H-2Kb, which stimulates specific secondary in vitro cytotoxic T lymphocyte (CTL) responses, could not replace cell-associated H-2Kb in the stimulation of these T-cell lines, even in the presence of feeder cells and interleukins. When T-cell lines were initiated in vitro and repeatedly stimulated with H-2Kb liposomes and feeder cells, it was possible to obtain T cells that could proliferate in response to H-2Kb liposomes in the presence of feeder cells and interleukin-2-containing supernatants or on H-2K b-expressing cells. Only stimulation with cells permitted maintenance of these T cells in culture for more than 12 weeks. Analyses of cell surface markers and of patterns of inhibition of proliferation by monoclonal antibodies (mAb) of T-cell lines induced in vitro with cell- or liposome-associated H-2Kb indicated that T-cell stimulation by class I antigen can occur in at least two ways. In the first, the H-2Kb-induced proliferation of Lyt-1- Lyt-2+ T4- T cells is inhibited by H-2Kb- and by Lyt-2-specific mAb, but not by Ia or T4-specific mAb. In the second, both Lyt-2+ and T4+ T cells are involved and the H-2Kb-induced proliferation is inhibited by H-2Kb- and Lyt-2-specific mAb and by Ia- and T4-specific mAb.

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Abbreviations

Ab:

antibody

mAb:

monoclonal antibody

C:

complement

i.p.:

intraperitoneally

PBS:

phosphate-buffered saline

PBS-B-N:

PBS containing bovine serum albumin and NaN3

CTL:

cytotoxic T lymphocyte

Th:

T helper cell

MHC:

major histocompatibility complex

PMA:

4β-phorbol 12β-myristate 13-acetate

SCA:

concanavalin A-stimulated rat spleen cell supernatant

SC16:

EL4 clone 16 supernatant

IL-1:

interleukin-1

IL-2:

interleukin-2 (T-cell growth factor)

FCS:

fetal calf serum

H-2Kb-lip.:

H-2Kb inserted in liposomes

C. E.:

cell equivalents

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Authors and Affiliations

  1. Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288, Marseille Cedex 9, France

    F. Albert, C. Boyer, M. Buferne & A. M. Schmitt-Verhulst

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  1. F. Albert
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  2. C. Boyer
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  3. M. Buferne
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  4. A. M. Schmitt-Verhulst
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Albert, F., Boyer, C., Buferne, M. et al. Interaction between MHC-encoded products and cloned T cells. Immunogenetics 19, 279–294 (1984). https://doi.org/10.1007/BF00345401

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  • DOI: https://doi.org/10.1007/BF00345401

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