Summary
At one month, the thymus weight of NMRI mice heterozygous for the Nu gene was lower than that of the wild type. However, the thymic structure was normal and no significant anomalies at the ultrastructural level could be found either in epithelial cells or in thymocytes. Stereologic data showed that the loss of weight was only due to a decrease of the lymphoid population, whereas the absolute number of epithelial cells was unmodified.
Several hypotheses that could explain the reduced number of thymocytes were discussed.
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References
Cordier, A.C.: Ultrastructure of the thymus in ‘nude’ mice. J. Ultrastruct. Res. 47, 26–40 (1974)
Cordier, A.C., Heremans, J.F.: Nude mouse embryo: ectodermal nature of the primordial thymic defect. Scand. J. Immunol. 4, 193–196 (1975)
Dardenne, M., Papiernik, M., Bach, J.F., Stutman, O.: Studies on thymus products. III. Epithelial origin of the serum thymic factor. Immunology 27, 299–304 (1974)
Fisher, R.A., Yates, F.: Statistical Tables for biological, agricultural and medical Research. London: Oliver and Boyd 1963
Kawabata, I., Paparella, M.: Atypical cilia in normal human and guinea pig middle ear mucosa. Acta Otolaryngol. (Stockh.) 67, 511–515 (1969)
Kindred, B., Loor, F.: Activity of host-derived T cells which differentiate in nude mice grafted with co-isogenic or allogeneic thymuses. J. Exp. Med. 139, 1215–1227 (1974)
Loor, F., Kindred, B.: Differentiation of T-cell precursors in nude mice demonstrated by immunofluorescence of T-cell membrane markers. J. Exp. Med. 138, 1044–1055 (1973)
Luft, J.H.: Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9, 409–414 (1961)
Lynch, N.R., Prin, J., Salomon, J.-C.: Passive cutaneous anaphylaxis and vasoactive amine challenge in nude (nu/nu) mice. A comparison with nu/+, axenic and young normal animals. Immunology 32, 89–94 (1977)
Mandel, T.: Differentiation of epithelial cells in the mouse thymus. Z. Zellforsch. 106, 498–515 (1970)
Metcalf, D.: The thymus. (144pp.). Berlin-Heidelberg-New York: Springer-Verlag 1966
Moore, M.A.S., Owen, J.J.T.: Experimental studies on the development of the thymus. J. Exp. Med. 126, 715–726 (1967)
Outzen, H.C., Custer, R.Ph., Eaton, G.J., Prehn, R.T.: Spontaneous and induced tumor incidence in germfree ‘nude’ mice. J. Reticuloendothel. Soc. 17, 1–9 (1975)
Pantelouris, E.M.: Absence of thymus in a mouse mutant. Nature 217, 370–371 (1968)
Pantelouris, E.M.: Observations on the immunobiology of ‘nude’ mice. Immunology 20, 247–252 (1971)
Pantelouris, E.M., Flisch, P.A.: Estimation of PFC and serum haemolysin response to SRBC in ‘nude’ mice. Immunology 22, 159–164 (1972)
Papiernik, M., Nabarra, B., Bach, J.F.: In vitro culture of functional human thymic epithelium. Clin. Exp. Immunol. 19, 281–287 (1975)
Pritchard, H., Micklem, H.S.: Immune responses in congenitally thymus-less mice. I. Absence of response to oxazolone. Clin. Exp. Immunol. 10, 151–161 (1972)
Pritchard, H., Micklem, H.S.: Haemopoietic stem cells and progenitors of functional T-lymphocytes in the bone marrow of ‘nude’ mice. Clin. Exp. Immunol. 14, 597–607 (1973)
Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Rygaard, J., Povlsen, C.O.: Heterotransplantation of a human malignant tumour to ‘nude’ mice. Acta Pathol. Microbiol. Scand. 77, 758–760 (1969)
Salomon, J.-C., Bazin, H.: Low levels of some serum immunoglobulin classes in nude mice. Rev. Eur. Etudes Clin. Biol. 17, 880–882 (1972)
Scheid, M.P., Goldstein, G., Boyse, E.A.: Differentiation of T cells in nude mice. Science 190, 1211–1213 (1975)
Scheiff, J.-M.: Multinucleate cells in thymus of C3H mice. Cell Tissue Res. 170, 305–314 (1976)
Scheiff, J.-M., Cordier, A.C.: Etude quantitative du thymus de la souris hétérozygote pour le gène NU. Bull. Assoc. Anat. (Nancy) 58, 1063–1071 (1974)
Scheiff, J.-M., Cordier, A.C., Haumont, S.: Epithelial cell proliferation in thymic hyperplasia induced by triiodothyronine. Clin. Exp. Immunol. 27, 516–521 (1977)
Sousa, M.A.B. de, Parrot, D.M.V., Pantelouris, E.M.: The lymphoid tissues in mice with congenital aplasia of the thymus. Clin. Exp. Immunol. 4, 637–644 (1969)
Trump, B.F., Smuckler, E.A., Benditt, E.P.: A method for staining epoxy sections for light microscopy. J. Ultrastruct. Res. 5, 343–348 (1961)
Watson, M.L.: Staining of tissue sections for electron microscopy with heavy metals. J. Biophys. Biochem. Cytol. 4, 475–478 (1958)
Weibel, E.R.: Stereological techniques for electron microscopic morphometry. In: Principles and techniques of electron microscopy. Biological applications, Vol. 3, pp. 237–296. New York and London: Van Nostrand Reinhold 1973
Weibel, E.R., Kistler, G.S., Scherle, W.R.: Practical stereological methods for morphometric cytology. J. Cell Biol. 30, 23–38 (1966)
Wortis, H.H.: Immunological responses of ‘nude’ mice. Clin. Exp. Immunol. 8, 305–317 (1971)
Wortis, H.H., Nehlsen, S., Owen, J.J.: Abnormal development of the thymus in ‘nude’ mice. J. Exp. Med. 134, 681–692 (1971)
Zipori, D., Trainin, N.: Defective capacity of bone marrow from nude mice to restore lethally irradiated recipients. Blood 42, 671–678 (1973)
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Research supported by grant 10.013 of the ‘Fonds de la Recherche Fondamentale Collective’ (Brussels)
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Scheiff, J.M., Cordier, A.C. & Haumont, S. The thymus of Nu/+Mice. Anat. Embryol. 153, 115–122 (1978). https://doi.org/10.1007/BF00343368
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DOI: https://doi.org/10.1007/BF00343368