Summary
A thermosensitive mutation ptsI1 in the gene coding for the Enzyme I of the PEP-phosphotransferase system pathway has been isolated. The mutants enzyme was shown to be sensitive to high temperature, but this effect is dependent on the ionic strength. The ptsI1 mutation was shown to belong to the previously described ctr locus. Following Lin (1970) it is proposed to retain the symbole ptsI for this locus.
Similar content being viewed by others
References
Anagnostopoulos, C., Spizizen, J.: Requirements for transformation in Bacillus subtilis. J. Bact. 81, 741–746 (1961)
Buttin, G.: Mécanismes régulateurs dans la biosynthèse des enzymes du métabolisme du galactose chez Escherichia coli K12. J. molec. Biol. 7, 164–182 (1963)
Dahl, R., Morse, H. G., Morse, M. L.: Carbohydrate transport and cyclic 3′,5′ adenosine monophosphate (cAMP) levels in a temperature sensitive phosphotransferase mutant of Escherichia. Molec. gen. Genet. 129, 1–10 (1974)
Delobbe, A., Haguenauer, R., Rapoport, G.: Studies on the transport of αmethyl-d-glucopyranoside in Bacillus subtilis 168. Biochimie 53, 1015–1021 (1971)
Demerec, M., Adelberg, E. A., Clark, A.J., Hartman, P. E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76 (1966)
Egan, J. B., Morse, M. L.: Carbohydrate transport in S. aureus. I.— Genetic and biochemical analysis of a transport mutation. Biochim. biophys. Acta. (Amst.) 97, 310–319 (1965)
Epstein, W., Jewett, S., Fox, C. F.: Isolation and mapping of phosphotransferase mutants in Escherichia coli. J. Bact. 104, 793–794 (1970)
Gay, P., Cordier, P., Marquet, M., Delobbe, A.: Carbohydrate metabolism and transport in Bacillus subtilis. A study of ctr mutations. Molec. gen. Genet. 121, 355–368 (1973)
Gay, P., Rapoport, G.: Etude des mutants dépourvus de fructose-1-phosphate-kinase chez B. subtilis. C. R. Acad. Sci. (Paris) 271, 374–377 (1970)
Kundig, W., Ghosh, S., Roseman, S.: Phosphate bound to histidine protein as an intermediate in a novel phosphotransferase system. Proc. nat. Acad. Sci. (Wash.) 52, 1067–1074 (1964)
Lepesant, J.-A., Dedonder, R.: Transport du saccharose chez B. subtilis. C. R. Acad. Sci. (Paris) 267, 1109–1112 (1968)
Lepesant, J.-A., Kunst, F., Lepesant-Kejzlarová, J., Dedonder, R.: Chromosomal location of mutations affecting sucrose metabolism in B. subtilis Marburg. Molec. gen. Genet. 118, 135–160 (1972)
Lin, E. C. C.: The genetics of bacterial transport systems. Ann. Rev. Genet. 4, 225–262 (1970)
Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3, 208–218 (1961)
Marquet, M., Wagner, M.-C., Dedonder, R.: Separation of components of the phosphoenol pyruvate glucose phosphotransferase system in Bacillus subtilis Marburg. Biochimie 53, 1131–1134 (1971)
Marquet, M., Wagner, M.-C., Delobbe, A., Gay, P., Rapoport, G.: Mise en évidence de systèmes de phosphotransférases dans le transport du glucose, du fructose et du saccharose chez B. subtilis. C. R. Acad. Sci. (Paris) 271, 449–452 (1970)
Monod, J.: Recherches sur la croissance des cultures bactériennes. Thèse (1942, Paris): Ed. Hermann (Paris).
Neidhardt, F. C., Gros, F.: Metabolic instability of the ribonucleic acid synthetised by Escherichia coli in the presence of chloromycetin. Biochim. biophys. Acta (Amst.) 25, 513–520 (1957)
Pascal, M., Kunst, F., Lepesant, J.-A., Dedonder, R.: Characterization of two sucrase activities in B. subtilis. Biochimie 53, 1059–1066 (1971)
Saier, M. H., Simoni, R. D., Roseman, S.: The physiological behaviour of Enzyme I and heat stable protein mutant of bacterial phosphotransferase system., J. biol. Chem. 245, 5870–5875 (1970)
Schaeffer, P., Ionesco, H., Ryter, A., Balassa, G.: La sporulation de Bacillus subtilis, étude génétique et physiologique des mécanismes de régulation des activités cellulaires chez les microorganismes, p. 553–563. Paris: Ed. Centre National de la Recherche Scientifique 1965
Slayman, C. W.: The genetic control of membrane transport. Current Topics in Membranes and Transport 4, 1–174 edit. by Bronner, F., Kleinzeller, A. New York: Academic Press 1973
Takahashi, I.: Transducing phages for B. subtilis. J. gen. Microbiol. 31, 211–217 (1963)
Tanaka, S., Lin, E. C. C.: Two classes of pleiotropic mutants of Aerobacter aerogenes lacking components of a phosphoenol-pyruvate phosphotransferase system. Proc. nat. Acad. Sci. (Wash.) 57, 913–919 (1967)
Tempest, D. W., Meers, J. L., Brown, C. M.: Influence of environment on the content and composition of microbial free amino acid pools. J. gen. Microbiol 64, 171–185 (1970)
Young, F. E., Smith, C., Reilly, B. E.: Chromosomal location of genes regulating resistance to bacteriophages in B. subtilis. J. Bact. 98, 1087–1097 (1969)
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Niaudet, B., Gay, P. & Dedonder, R. Identification of the structural gene of the PEP-phosphotransferase enzyme I in Bacillus subtilis marburg. Molec. gen. Genet. 136, 337–349 (1975). https://doi.org/10.1007/BF00341718
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00341718