Summary
Cloned restriction fragments from wheat chloroplast DNA have been shown to direct the synthesis, in a cell-free E. coli transcription-translation system, of the beta and epsilon subunits of the CF1 component of ATP synthase. The products were identified by immunoprecipitation, their mobility in SDS-polyacrylamide gels and peptide mapping of limited proteolytic digests. Genes for these subunits have been located in the large single-copy region of wheat chloroplast DNA, very close to the gene for the large subunit of ribulose bisphosphate carboxylase.
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Howe, C.J., Bowman, C.M., Dyer, T.A. et al. Localization of wheat chloroplast genes for the beta and epsilon subunits of ATP synthase. Mol Gen Genet 186, 525–530 (1982). https://doi.org/10.1007/BF00337960
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DOI: https://doi.org/10.1007/BF00337960