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Effects of the herbicide imazethapyr on soil microbial biomass and various soil enzyme activities

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Abstract

The effects of imazethapyr, an imidazolinone derivative on the soil microbial biomass was investigated in a clay loam soil. Imazethapyr was applied in a field trial at the recommended field rate for soybean weeding, 50 g a.i. ha-1. In laboratory experiments the herbicide was incorporated at the field rate and 10-fold and 100-fold higher. In both the field trials and the laboratory experiments, the field rate of imazethapyr had no adverse effects on the microbiological processes tested. But at 10-fold and 100-fold higher rates, the herbicide decreased the biomass-C content and dehydrogenase activity and increased the hydrolytic capacity and protease and catalase activity. These findings suggest that there may be a risk in monocultural practices that require repeated herbicide treatments.

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References

  • Barrett M (1989) Protection of corn (Zea mays) and sorghum (Sorghum bicolor) from imazethapyr toxicity with antidotes. Weed Sci 37:296–299

    Google Scholar 

  • Beyer EM, Duffy MJ, Hay JV, Schlueter DD (1988) Sulfonylureas. In: Kearney PC, Kaufmann DD (eds) Herbicides — chemistry, degradation and mode of action. Dekker, New York Basel, pp 117–189

    Google Scholar 

  • Ladd JN (1985) Soil enzymes. In: Vaughan D, Malcolm RE (eds) Soil Organic matter and biological activity. Martinus Nijhoff/Junk Publishers, Dordrecht Boston Lancaster, pp 175–221

    Google Scholar 

  • Ladd JN, Butler JHA (1972) Short-term assays of soil proteolytic enzyme activities using proteins and dipeptide derivatives as substrates. Soil Biol Biochem 4:19–30

    Google Scholar 

  • Le Clerg EL, Leonard WH, Clark AG (1962) Field plot technique. Burges Publishing Co, Minneapolis, Minn

    Google Scholar 

  • Roberge MR (1978) Methodology of soil enzyme measurement and extraction. Appendix on soil enzymes. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 341–370

    Google Scholar 

  • Rossell D, Maire N, Tarradellas J (1992) Dehydrogenase activity and biomass soil microflora. Proceedings of the international symposium on environmental aspects of pesticide microbiology. Sigtuna, p 48

  • Shaner DL, O'Connor SL (1991) The imidazolinone herbicides. CRC Press, Boca Raton Ann Arbor Boston London

    Google Scholar 

  • Società Italiana Scienza del Suolo (1985) Metodi normalizzati di analisi del suolo. Edagricole, Bologna

    Google Scholar 

  • Sparling GP, West AW (1988) A direct extraction method to estimate soil microbial C: Calibration in situ using microbial respiration and 14C labelled cells. Soil Biol Biochem 20:337–343

    Google Scholar 

  • Swisher R, Carroll GC (1980) Fluorescein diacetate hydrolysis as an estimator of microbial biomass and coniferous needle surfaces. Microb Ecol 6:217–226

    Google Scholar 

  • Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass. Soil Biol Biochem 19:703–707

    Google Scholar 

  • Vekemans X, Godden B, Pennickx MJ (1989) Factor analysis of the relationship between several physicochemical and microbial characteristics of some Belgian agricultural soils. Soil Biol Biochem 21:53–58

    Google Scholar 

  • von Mersi W, Schinner F (1991) An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol Fertil Soils 11:216–220

    Google Scholar 

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Research supported by National Research Council of Italy, Special Project RAISA, Subproject no. 2, Paper no. 1105

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Perucci, P., Scarponi, L. Effects of the herbicide imazethapyr on soil microbial biomass and various soil enzyme activities. Biol Fertil Soils 17, 237–240 (1994). https://doi.org/10.1007/BF00336329

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  • DOI: https://doi.org/10.1007/BF00336329

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