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Stratification within centrifuged amoeba nuclei

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Summary

Two species of large, fresh water amoebae were ultracentrifuged and studied with the electron microscope. Emphasis was placed on the stratification of the nucleoplasm, including nucleoli, within the confines of the nuclear envelope during interphase. Three major strata were found in the nuclei of both amoeba species, namely the centripetal nucleoplasm, the middle chromatin stratum, and the centrifugal nucleolar mass. In the highly radioresistant A. proteus, the nucleolar mass separated into a centripetal electron-opaque layer and a centrifugal electron-lucent layer. The latter layer appears to be missing from the radiosensitive P. illinoisensis. The nature of these nucleolar layers and their possible relationship to differences in radiosensitivity between the two species of amoebae is discussed. The contents of the heavier of the two nucleolar layers in A. proteus might be resistant to radiation damage and may possess radiorestorative capacity.

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References

  • Beams, H. W.: Effects of ultracentrifugation on the structure of Ehrlich ascites tumor cells. Anat. Rec. 151, 322 (1965).

    Google Scholar 

  • —, J. B. Gatenby, and J. A. Muliyil: Ultra-centrifuging the spermatocytes of Helix aspersa. Quart. J. micr. Sci. 78, 387–396 (1936).

    Google Scholar 

  • —, and R. G. Kessel: Ultracentrifugation and electron microscope studies on Ehrlich ascites tumor cells. Amer. Zool. 2, 390 (1962).

    Google Scholar 

  • —, and R. L. King: The effect of ultracentrifuging upon chick embryonic cells, with special reference to the “resting” nucleus and the mitotic spindle. Biol. Bull. 71, 188–198 (1936).

    Google Scholar 

  • —: The effect of centrifugation on plant cells. Bot. Rev. 5, 132–154 (1939).

    Google Scholar 

  • —, T. N. Tahmisian, E. Anderson, and R. Devine: Studies on the fine structure of ultracentrifuged spinal ganglion cells. J. biophys. biochem. Cytol. 8, 793–811 (1960).

    Google Scholar 

  • Birnstiel, M. L., M. I. H. Chipchase, and B. B. Hyde: The nucleolus, a source of ribosomes. Biochim. biophys. Acta (Amst.) 76, 454–462 (1963).

    Google Scholar 

  • —, J. L. Sirlin, and J. Jacob: The nucleolus: a site of transfer ribonucleic acid synthesis. Biochem. J. 94, 10P-11P (1965).

    Google Scholar 

  • Bouck, G. B.: Stratification and subsequent behavior of plant cell organelles. J. Cell Biol. 18, 441–457 (1963).

    Google Scholar 

  • Busch, H., P. Byvoet, and K. Smatana: The nucleolus of the cancer cell: A review. Cancer Res. 23, 313–339 (1963).

    Google Scholar 

  • Comb, D. G., R. Brown, and S. Katz: The nuclear DNA and RNA components of the aquatic fungus Blastocladiella emersonii. J. Mol. Biol. 8, 781–789 (1964).

    Google Scholar 

  • —, and S. Katz: Studies on the biosynthesis and methylation of transfer RNA. J. molec. Biol. 8, 790–800 (1964).

    Google Scholar 

  • Costello, D. P.: The fertilizability of nucleated and non-nucleated fragments of centrifuged Nereis eggs. J. Morph. 66, 99–114 (1940).

    Google Scholar 

  • Daniels, E. W., and E. P. Breyer: Fine structure of centrifuged amoeba nuclei. Div. Biol. Med. Res., Argonne Nat. Lab. Ann. Rpt. No. ANL-6971, 62–63 (1964a).

  • —, and C. W. Kischer: Fine structure of centrifuged amoeba nuclei. J. Protozool. (Suppl.) 11, 13–14 (1964b).

    Google Scholar 

  • - - - Intranuclear stratification in the amoeba, Pelomyxa illinoisensis. Div. Biol. Med. Res., Argonne Nat. Lab. Rpt. No. ANL-6823, 169–173 (1964c).

  • Edstrom, J. E.: Composition of ribonucleic acid from various parts of spider oocytes. J. biophys. biochem. Cytol. 8, 47–51 (1960).

    Google Scholar 

  • —, W. Grampp, and N. Schor: The intracellular distribution and heterogeneity of ribonucleic acid in starfish oocytes. J. biophys. biochem. Cytol. 11, 549–557 (1961).

    Google Scholar 

  • Katzberg, A. A., and G. T. Daye: Survival of the isolated embryonic heart when exposed to high relative centrifugal forces. Sch. Aviation Med., USAF Aerospace Med. Center (ATC), Brooks AFB, Texas Rpt. No. AF-SAM-61-19, 1–9 (1960).

  • Kopac, M. J., and G. M. Mateyko: Nucleolar chromosomes: Structures, interactions, and perspectives. Advanc. Cancer Res. 8, 121–190 (1964).

    Google Scholar 

  • Kudo, R. R.: A species of Pelomyxa from Illinois. Trans. Amer. Microscop. Soc. 69, 368–370 (1950).

    Google Scholar 

  • —: Observations on Pelomyxa illinoisensis. J. Morph. 88, 145–184 (1951).

    Google Scholar 

  • Lafontaine, J. G., and L. A. Chouinard: A correlated light and electron microscope study of the nucleolar material during mitosis in Vicia faba. J. Cell Biol. 17, 167–201 (1963).

    Google Scholar 

  • Lerman, M. I., V. L. Mant'eva, and G. P. Georgiev: Biosynthesis of ribosomal RNA in the nucleolus (nucleonemal apparatus of the cell). Biokhimiya 29, 518–528 (1964).

    Google Scholar 

  • McClellan, J. F.: Nuclear division in Pelomyxa illinoisensis Kudo. J. Protozool. 6, 322–331 (1959).

    Google Scholar 

  • Mercer, E. H.: An electron microscopic study of Amoeba proteus. Proc. roy. Soc., Sev. B 150, 216–232 (1959).

    Google Scholar 

  • Mottier, D. M.: The effect of centrifugal force upon the cell. Ann. Bot. 13, 325–362 (1899).

    Google Scholar 

  • Muramatsu, M., J. L. Hodnett, and H. Busch: Studies on the “independence” of nucleolar ribonucleic acid synthesis. Biochim. biophys. Acta (Amst.) 91, 592–597 (1964).

    Google Scholar 

  • Özban, N., C. J. Tandler, and J. L. Sirlin: Methylation of nucleolar RNA during development of the amphibian oocyte. J. Embryol. exp. Morph. 12, 373–380 (1964).

    Google Scholar 

  • Pappas, G. D.: Helical structures in the nucleus of Amoeba proteus. J. biophys. biochem. Cytol. 2, 221–222 (1956).

    Google Scholar 

  • —: Electron microscope studies on amoebae. Ann. N. Y. Acad. Sci. 78, 448–473 (1959).

    Google Scholar 

  • Perry, R. P.: Role of the nucleolus in ribonucleic acid metabolism and other cellular processes. Nat. Cancer Inst. Monogr. 14, 73–87 (1964).

    Google Scholar 

  • Rafalko, J. S.: A modified Feulgen technic for small and diffuse chromatin elements. Stain Technol. 21, 91–93 (1946).

    Google Scholar 

  • Roth, L. E., S. W. Obetz, and E. W. Daniels: Electron microscopic studies of mitosis in amoebae. I. Amoeba proteus. J. biophys. biochem. Cytol. 8, 207–220 (1960).

    Google Scholar 

  • Sirlin, J. L.: The nucleolus. Progess in Biophysics and Biophysical Chemistry, vol. 12, p. 27–66, 319–327 (sEd. J. A. V. Butler, H. E. Huxley, and R. E. Zirkle). New York: Pergamon Press 1962.

    Google Scholar 

  • —, and J. Jacob: Sequential and reversible inhibition of synthesis of ribonucleic acid in the nucleolus and chromosomes: Effect of benzamide and substituted benzimidazoles on dipteran salivary glands. Nature (Lond.) 204, 545–547 (1964).

    Google Scholar 

  • Stevens, B. J.: The effect of actinomycin D on nucleolar and nuclear fine structure in the salivary gland cell of Chironomus thummi. J. Ultrastruct. Res. 11, 329–353 (1964).

    Google Scholar 

  • Swift, H.: Studies on nucleolar function. Symp. Mol. Biol. (R. E. Zirkle, sed.), p. 266–303. Chicago: Chicago University Press. 1959.

    Google Scholar 

  • Thomson, J. F., and E. W. Daniels: Cytochrome oxidase in radiosensitive and radioresistant amoebae. Proc. Soc. exp. Biol. and Med. (N. Y.) 107, 916–919 (1961).

    Google Scholar 

  • Vincent, W. S.: Heterogeneity of nuclear ribonucleic acid. Science 126, 306–307 (1957).

    Google Scholar 

  • —, and E. Baltus: A function for the nucleolus. Biol. Bull. 119, 299–300 (1960).

    Google Scholar 

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Authors and Affiliations

  1. Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois, USA

    Edward W. Daniels & Evelyn P. Breyer

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  1. Edward W. Daniels
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  2. Evelyn P. Breyer
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Work supported by U.S. Atomic Energy Commission. A part of the work was reported at the 17th Meeting of the Society of Protozoologists, Boulder, Colorado in 1964.

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Daniels, E.W., Breyer, E.P. Stratification within centrifuged amoeba nuclei. Zeitschrift für Zellforschung 70, 449–460 (1966). https://doi.org/10.1007/BF00335354

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  • DOI: https://doi.org/10.1007/BF00335354

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