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Sub-families of hordein mRNA encoded at the Hor 2 locus of barley

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Summary

Previous studies indicated that the Hor2 locus of barley, which specifies B hordein, is a complex family of genes derived from the duplication and divergence of a single ancestral gene. In vitro translation of poly A+ RNA from developing endosperms of seven cultivars shows that the hordein polymorphism is not due to post-translational modification of a few polypeptides. Each polypeptide appears to be specified by a separate mRNA and presumably a separate gene. Cloned cDNAs derived from B hordein mRNAs were used to select mRNAs from endosperms of seven barley cultivars with different Hor2 alleles. Comparison of the products of translation of these hybrid-selected mRNAs indicated the presence of two sub-families of B hordein mRNAs which code for different classes of hordein polypeptides previously recognized on the basis of cyanogen bromide peptide mapping. The proportions of the mRNAs for the two sub-families of B hordein polypeptides appeared to be correlated with the amounts of the polypeptides present in the endosperms.

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Authors and Affiliations

  1. Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Hertfordshire, UK

    M. Kreis, S. Rahman, B. G. Forde, J. Pywell, P. R. Shewry & B. J. Miflin

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  1. M. Kreis
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  2. S. Rahman
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  3. B. G. Forde
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  4. J. Pywell
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  5. P. R. Shewry
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  6. B. J. Miflin
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Communicated by J. Schell

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Kreis, M., Rahman, S., Forde, B.G. et al. Sub-families of hordein mRNA encoded at the Hor 2 locus of barley. Mol Gen Genet 191, 194–200 (1983). https://doi.org/10.1007/BF00334813

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  • DOI: https://doi.org/10.1007/BF00334813

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