Summary
The pancreatic islets of diabetic (KK) and control (C57 BL/6) strains of the mouse have been studied by histochemical methods for the demonstration of a series of hydrolyzing enzymes, and the following results were obtained.
-
1.
In KK mice alkaline phosphatase activity is demonstrated in fewer insular cells and more vascular walls than in control animals.
-
2.
The islet cells of older KK mice exhibiting persistent glucosuria react for acid phosphatase less intensely than those of control animals.
-
3.
In the islet cells of KK animals glucose-6-phosphatase activity is significantly higher than that revealed by the cells of control mice.
-
4.
The islet cells of both the KK and control mice exhibit moderate ATP-ase activity, and there is no strain difference in the morphological features of the enzyme activity.
-
5.
All the present enzymorphological data were discussed with special reference to the presumed metabolic disturbance in the diabetic (KK) mice.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dempsey, E., R. Greep, and H. Deane: Changes in the distribution and concentration of alkaline phosphatase in tissues of the rat after hypophysectomy or gonadectomy, and replacement therapy. Endocrinology 44, 88–103 (1949).
Gössner, W.: Histochemischer Nachweis hydrolytischer Enzyme mit der Azofarbstoffmethode, Untersuchungen zur Methodik und vergleichenden Histotopik der Esterasen und Phosphatasen bei Wirbeltieren. Histochemie 1, 48–96 (1958).
Gomori, G.: The distribution of phosphatase in normal organs and tissues. J. cell. comp. Physiol. 17, 71–83 (1941).
—: An improved histochemical technic for acid phosphatase. Stain Technol. 25, 81–85 (1950).
—: Microscopic Histochemistry. Chicago: University of Chicago Press 1952.
Hellman, B., and C. Hellerström: Histochemical studies on glucose-6-phosphatase, adenosine triphosphatase and amylophosphorylase in the pancreatic islets of normal and obesehyperglycemic mice. Acta endocr. (Kbh.) 39, 474–482 (1962).
—, S. Larsson, and S. Brolin: Histochemical observations on the pancreatic islets in normal and obese-hyperglycemic mice. Z. Zellforsch. 55, 235–246 (1961).
Lazarus, S.: Demonstration of glucose-6-phosphatase in mammalian pancreas. Proc. Soc. exp. Biol. Med. 101, 819–822 (1959a).
—: Acid and glucose-6-phosphatase activity of pancreatic B cells after cortisone and sulforyl-urea. Proc. Soc. exp. Biol. Med. 102, 303–306 (1959b).
—, and B. Volk: The pancreas in human and experimental diabetes. New York and London: Grune & Stratton 1962.
Nakamura, M.: A diabetic strain of the mouse. Proc. Japan Acad. 38, 348–352 (1962).
—: Cytological and histological studies on the pancreatic islets of a diabetic strain of the mouse. Z. Zellforsch. 65, 340–349 (1965).
Noback, C. R., and W. Montagna: Histochemical studies of the basophilia, lipase and phosphatase in the mammalian pancreas and salivary glands. Amer. J. Anat. 81, 343–364 (1947).
Novikoff, A. B., L. Hecht, E. Podber, and J. Ryan: Phosphatase of rat liver: I. The dephosphorylation of adenosine-triphosphate. J. biol. Chem. 194, 153–170 (1952).
Padykula, H. A., and E. Herman: Factors affecting activity of adenosine triphosphatase and other phosphatases as measured by histochemical techniques. J. Histochem. Cytochem. 3, 161–169 (1955a).
—: Specificity of histochemical method for adenosine-triphosphatase. J. Histochem. Cytochem. 3, 170–195 (1955b).
Pearce, A. G. E.: Histochemistry. London: Churchill 1960.
Staats, J.: Standardized nomenclature for inbred strains of mice, third listing. Cancer Res. 24, 147–168 (1964).
Täljedal, I., B. Hellman, and C. Hellerström: Quantitative studies on isolated pancreatic islets of mammals: adenosine:triphosphatase activity in normal and obese-hyperglycemic mice. J. Histochem. Cytochem. 12, 491–497 (1964).
Uematsu, H., and T. Kawasumi: Paperchromatography on the enzymatic dephosphorylation products of adenosine-triphosphate. J. Histochem. Cytochem. 11, 665 (1963).
Vorbrodt, A.: Histochemically demonstrable phosphatases and protein synthesis. Exp. Cell. Res. 15, 1–20 (1958).
Wachstein, M.: Histochemical staining reactions of the normally functioning and abnormal kidney. J. Histochem. Cytochem. 3, 246–270 (1956).
Author information
Authors and Affiliations
Additional information
Acknowledgements. The authors express their appreciation to Profs. Kazumaro Yamada and Jun hara for the constant supervision and encouragement during the course of this study.
Rights and permissions
About this article
Cite this article
Nakamura, M., Yamada, K. Enzymorphological studies on the pancreatic islets of a diabetic (KK) strain of the mouse. Zeitschrift für Zellforschung 66, 396–404 (1965). https://doi.org/10.1007/BF00334721
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00334721