Summary
The cytochemical localization of acid phosphatase and thiamine pyrophosphatase activity was studied by light and electron microscopy in prothoracic gland cells of the cockroach Leucophaea moderae. Nymphal and young adult animals were used.
Prominent sites of acid phosphatase activity included large membrane-bounded dense bodies or lysosomes, and certain cisternae of the Golgi apparatus. The results suggest a possible difference in the enzymatic activity toward glycerophosphate and aromatic phosphates as substrates.
Thiamine pyrophosphatase activity was localized in elements of the Golgi apparatus and endoplasmic reticulum, and in lysosome-like dense bodies. This latter activity was abolished by sodium fluoride treatment, whereas the phosphatase activity in the Golgi apparatus and endoplasmic reticulum is unaffected by such inhibition.
The cytochemical results confirm through direct evidence the suggestions of Scharrer (1964), that the large dense bodies present in the prothoracic gland cells are lysosomes, and that their activity may be related to stages in the life history of the glands. Furthermore, the lysosomes or their derivative structures may play an essential role in the autolysis of the prothoracic glands toward the end of their active period.
The enzymatic activity of the endoplasmic reticulum may indicate the involvement of this organelle in the metabolism of “steroid-like” precursor materials necessary for the synthesis of ecdysone.
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References
Beaulaton, J.: Évolution du chondriome dans la glande prothoracique du ver à soie tussor (Antheraea Pernyi guér). au cours du cycle sécrétoire pendant les quatrième et cinquième stades larvaires. J. Microscopie 3, 167–186 (1964).
Burstone, M. S.: Histochemical demonstration of acid phosphatase with naphthol AS-phosphates. J. nat. Cancer Inst. 21, 523–539 (1958).
Christensen, A. K.: The fine structure of testicular interstitial cells in guinea pigs. J. Cell Biol. 26, 911–936 (1965).
Duve, C. de: From cytase to lysosomes. Fed. Proc. 23, 1045–1049 (1964).
Goldfischer, S., E. Essner, and A. B. Novikoff: The localization of phosphatase activities at the level of ultrastructure. J. Histochem. Cytochem. 12, 72–95 (1964).
Gomori, G.: Microscopic histochemistry. Chicago: Chicago University Press 1952.
Hayano, M., and R. I. Dorfman: Enzymes of steroid hydroxylation. In: Methods in enzymology, ed. by S. P. Colowick and N. O. Kaplan, vol. V, p. 503–512. New York: Academic Press 1962.
Hirshfield, I. N., and S. B. Koritz: Pregnenolone synthesis stimulation in the large particles from bovine adrenal cortex and bovine corpus luteum. Endocrinology 78, 165–169 (1966).
Jurand, A.: Ultrastructural aspects of early development of the fore-limb buds in the chick and the mouse. Proc. roy. Soc. B 162, 387–405 (1965).
Karlson, P.: The chemistry and biochemistry of ecdysone. Proc. XVI Internat. Congr. Zool., Washington 4, 221–225 (1963).
—, u. H. Hoffmeister: Zur Biogenese des Ecdysons, I. Umwandlung von Cholesterin in Ecdyson. Hoppe-Seylers Z. physiol. Chem. 331, 298–300 (1963).
Läufer, H.: Blood proteins in insect development. Ann. N. Y. Acad. Sci. 89, 490–515 (1961).
Lockshin, R. A., and C. M. Williams: Programmed cell death. I. Cytology of degeneration in the intersegmental muscles of the Pernyi silkmoth. J. Insect Physiol. 11, 123–133 (1965 a).
—: Programmed cell death. V. Cytolytic enzymes in relation to the breakdown of the intersegmental muscles of silkmoths. J. Insect Physiol. 11, 831–844 (1965b).
Meek, G. A., and N. J. Lane: The ultrastructural localization of phosphatases in the neurones of the snail, Helix aspersa. J. roy. micr. Soc., Ser. III 82, 193–204 (1964).
Misch, D. W.: Localization of acid phosphatase in tissues of metamorphosing flesh-fly larvae. J. Histochem. Cytochem. 10, 666 (1962).
Novikoff, A. B.: Lysosomes and related perticles. In: The cell, ed. by J. Brachet and A. E. Mirsky, vol. 2, p. 423–488. New York: Academic Press 1961.
—: Lysosomes in the physiology and pathology of cells: contributions of staining methods. In: Lysosomes, ed. by A.V.S. de Rueck and M. P. Cameron, Ciba Found. Symp., p. 36–77. Boston: Little, Brown & Co. 1963.
—, E. Essner, S. Goldfischer, and M. Heus: Nucleosidephosphatase activities of cytomembranes. Symp. Intl. Soc. Cell Biol. 1, 149–192 (1962).
—, and S. Goldfischer: Nucleosidediphosphate activity in the Golgi apparatus and its usefulness for cytological studies. Proc. nat. Acad. Sci. (Wash.) 47, 802–810 (1961).
Osinchak, J.: Electron microscopic localization of acid phosphatase and thiamine pyrophosphatase activity in hypothalamic neurosecretory cells of the rat. J. Cell Biol. 21, 35–47 (1964).
—: The electron microscopic localization of acid phosphatase and thiamine pyrophosphatase activity in prothoracic glands of the insect Leucophaea maderae. Anat. Rec. 151, 395 (1965).
Rasch, E. M., and S. Gawlik: Cytolysomes in tissues of metamorphosing sciarid larvae. J. Cell Biol. 23, 123 A (1964).
Reynolds, E. S.: The use of lead citrate ot high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Rosenbaum, R. M., and C. I. Rolon: Species variability and the substrate specificity of intracellular acid phosphatases: A comparison of the lead-salt and azo-dye methods. Histochemie 3, 1–16 (1962).
Sabatini, D. D., K. Bensch, and R. J. Barrnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell. Biol. 17, 19–58 (1963).
Scharrer, B.: The fine structure of the blattarian prothoracic glands. Z. Zellforsch. 64, 301–326 (1964).
—: An ultrastructural study of cellular regression as exemplified by the prothoracic gland of Leucophaea maderae. Anat. Rec. 151, 411 (1965).
—: Ultrastructural study of the regressing prothoracic glands of blattarian insects. Z. Zellforsch. 69, 1–21 (1966).
Scheib, D.: Properties and role of acid hydrolases of the Müllerian ducts during sexual differentiation in the male chick embryo. In: Lysosomes, ed. by A. V. S. de Rueck and M. P. Cameron, Ciba Found. Symp., p. 264–277. Boston: Little, Brown & Co. 1963.
—: Sur la régression du canal de Müller mâle de l'embryon de poulet: localisation de la phosphatase acide au microscope électronique. C. R. Acad. Sci. Paris 261, 5219–5221 (1965).
Schin, K. S., and U. Clever: Lysosomal and free acid phosphatase in salivary glands of Chironomus tentans. Science 150, 1053–1055 (1965).
Terner, J. Y., R. M. Goodman, and D. Spiro: Glucose-6-phosphatase in the salivary glands of Sciara coprophila: A histochemical and biochemical study. J. Histochem. Cytochem. 13, 168–181 (1965).
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This study was supported by U.S.P.H.S. grants 5 T1-MH-6418 and NB-05219, and grant RO 1-AM-3984 to Dr. Berta Scharrer. I would like to express my appreciation to Dr. Scharrer for her encouragement and assistance during this study. I also wish to thank Mrs. Sarah Wurzelmann for her competent technical aid.
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Osinchak, J. Ultrastructural localization of some phosphatases in the prothoracic gland of the insect Leucophaea Maderae . Z. Zellforsch. 72, 236–248 (1966). https://doi.org/10.1007/BF00334277
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DOI: https://doi.org/10.1007/BF00334277