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Behaviour of the transposons Tn5 and Tn7 in Xanthomonas campestris pv. campestris

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Summary

Xanthomonas campestris pv. campestris was tested for its ability to maintain various plasmids after they had been transferred by conjugation from Escherichia coli donors. Broad host-range plasmids belonging to incompatibility groups P and Q could be maintained but X. campestris was unable to support replication of narrow host-range ColE1, pACYC184 and pBR325 replicons. Delivery systems based on E. coli donors of suicide plasmids and on X. campestris Hfrs were used to introduce Tn7 and Tn5 into X. campestris. Tn7 insertions were recovered at high frequency while Tn5 transposed at low frequency. Three auxotrophic Tn5 insertions were isolated but transposition of Tn7 into the X. campestris genome did not generate any auxotrophs. DNA hybridization analysis showed that Tn7 had inserted into the same “hot spot(s)” in all cases tested.

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Authors and Affiliations

  1. John Innes Institute, NR4 7UH, Colney Lane, Norwich, England

    Peter Turner, Christine Barber & Michael Daniels

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  1. Peter Turner
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  2. Christine Barber
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  3. Michael Daniels
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Communicated by P. T. Emmerson

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Turner, P., Barber, C. & Daniels, M. Behaviour of the transposons Tn5 and Tn7 in Xanthomonas campestris pv. campestris . Mol Gen Genet 195, 101–107 (1984). https://doi.org/10.1007/BF00332731

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  • DOI: https://doi.org/10.1007/BF00332731

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