Summary
Plasmid-like, linear DNAs were detected in preparations of mitochondrial DNA from cytoplasmic male-sterile sorghum. Designated N-1 and N-2, the DNAs exhibited molecular sizes of ca. 5,700 and 5,300 bp, respectively. The DNAs occurred in only the IS1112C entry among 24 entries examined. Electron microscopy of the DNAs indicated that the molecules were linear as isolated. Nick translation of N-1 and N-2 followed by membrane hybridization indicated substantial homology between the two DNAs, and some homology to the S-1 and S-2 maize plasmid-like DNAs. At least four additional DNA species, ranging from ca. 1,000–4,000 bp if linear, were also detected in sorghum mitochondrial DNA. The detection of plasmid-like DNAs in sorghum, with homology to the plasmid-like DNAs of maize, suggests an etiological relationship of the molecules to the inheritance and expression of cytoplasmic male sterility in sorghum, perhaps in a manner analogous to the postulated role of these elements in maize.
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Conde MF, Pring DR, Schertz KF, Ross W (1982) Correlation of mitochondrial DNA restriction endonuclease patterns with sterility expression in six male-sterile sorghum cytoplasms. Crop Science (in press)
Daniels DL, de Wet JR, Blattner JR (1980) New map of bacteriophage lambda DNA. J Virol 33:390–400
Denhardt DT (1966) A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646
Gengenbach BG, Connelly JA, Pring DR, Conde MF (1981) Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theor Appl Genet 59:161–167
Jeffreys AJ, Flavell RA (1977) A physical map of the DNA regions flanking the rabbit B-globin gene. Cell 12:429–439
Kemble RJ, Bedbrook JR (1980) Low molecular weight circular and linear DNA in mitochondria from normal and male- sterile Zea mays cytoplasm. Nature 284:565–566
Kim BD, Mans RJ, Conde MF, Pring DR, Levings CSIII (1982) Physical mapping of homologous segments of mitochondrial episomes from S male-sterile maize. Plasmid 7:1–14
Laughnan JR, Gabay SJ (1973) Mutations leading to nuclear restoration of fertility in S male-sterile cytoplasm in maize. Theor Appl Genet 43:109–116
Laughnan JR Gabay SJ (1975) An episomal basis for instability of S male sterility in maize and some implications for plant breeding. In: Birky CW, Perlman Jr PS, Byers TJ (eds) Genetics and biogenesis of mitochondria and chloroplasts. Ohio State Univ Press, Columbus, p 330
Laughnan JR, Gabay SJ (1978) Nuclear and cytoplasmic mutations to fertility in S male-sterile maize, In: Walden DB (ed), Maize breeding and genetics. John Wiley and Sons, New York, p 427
Levings CSIII, Kim BD, Pring DR, Conde MF, Mans PJ, Laughnan JR, Gabay-Laughnan SJ (1980) Cytoplasmic reversion of Cms-S in maize is associated with a transpositional event. Science 209:1021–1023
Lonsdale DM, Thompson RD, Hodge TP (1981) The integrated forms of the S1 and S2 DNA elements of maize male sterile mitochondrial DNA are flanked by a large repeated segment. Nucl Acid Res 9:3657–3669
Maniatis TA, Jeffery A, Kleid DG (1975) Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci USA 72:1184–1188
Powling A (1981) Species of small DNA molecules found in mitochondria from sugarbeet with normal and male-sterile cytoplasms. Mol Gen Genet 183:82–84
Pring DR, Conde MF, Schertz KF (1982) Organelle genome diversity in sorghum: male-sterile cytoplasms. Crop Science (in press)
Pring DR, Levings CSIII (1978) Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89:121–136
Pring DR, Levings CSIII, Hu WWL, Timothy DH (1977) Unique DNA associated with mitochondria in the “S”-type cytoplasm of male-sterile maize. Proc Natl Acad Sci USA 74:2904–2908
Pring DR, Schertz KF, Conde MF (1981) Plasmid-like and other cytoplasmic DNA characteristics of male-sterile sorghum. Sorghum Newsletter 24:132
Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes JC, Hutchinson CAIII, Slocombe PM, Smith M (1977) Nucleotide sequence of bacteriophage X174 DNA. Nature 265:687–695
Sederoff RR, Levings CSIII, Timothy DH, Hu WWL (1981) Evolution of DNA sequence organization in mitochondrial genomes of Zea. Proc Natl Acad Sci USA 78:5953–5957
Singh A, Laughnan JR (1972) Instability of S male-sterile cytoplasm in maize. Genetics 71:607–620
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Spruill WM Jr, Levings CSIII, Sederoff RR (1980) Recombinant DNA analysis indicates that the multiple chromosomes of maize mitochondria contain different sequences. Devel Genetics 1:363–388
Thompson RD, Kemble RJ, Flavell RB (1980) Variations in mitochondrial organization between normal and male-sterile cytoplasms of maize. Nucl Acids Res 8:1999–2008
Wessinger AK, Timothy DH, Levings CSIII, Hu WWL, Goodman MM (1982) Unique plasmid-like mitochondrial DNAs from indigenous maize races of Latin America. Proc Natl Acad Sci USA 79:1–5
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Communicated by H. Saedler
Cooperative Investigations of Agricultural Research Service, U.S. Department of Agriculture, Institute of Food and Agricultural Sciences, University of Florida, Texas Agricultural Experiment Station, and Department of Genetics, North Carolina State University. Florida Experiment Stations Journal Series No. 3578
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Pring, D.R., Conde, M.F., Schertz, K.F. et al. Plasmid-like DNAs associated with mitochondria of cytoplasmic male-sterile Sorghum . Mol Gen Genet 186, 180–184 (1982). https://doi.org/10.1007/BF00331848
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DOI: https://doi.org/10.1007/BF00331848