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Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli

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Summary

The nucleotide sequence of the gene encoding the glycolytic enzyme phosphoglucose isomerase (PGI) from Escherichia coli is presented. The gene encodes a polypeptide of 549 amino acids. The transcriptional start point of the gene was determined and found to lie within a consensus promoter region. The amino acid sequence derived from the E. coli PGI gene can be aligned without insertions or deletions to the predicted amino acid sequence of a nuclear-encoded chloroplast isozyme of PGI from a higher plant, and the two sequences have a similarity of 87.6%. The amino acid sequence similarity between E. coli and that predicted from cDNA sequences for mouse and pig PGI is approximately 65%.

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Abbreviations

Ap:

ampicillin

aa:

amino acid(s)

nt:

nucleotide(s)

ORF:

open reading frame

PGI:

phosphoglucose isomerase

MW:

molecular weight

kDa:

kilo Daltons

ss:

single strand

RF:

replicative form

LB:

Luria broth

kb:

kilobasepairs

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Authors and Affiliations

  1. Department of Genetics, University of California, 95616, Davis, CA, USA

    Byron E. Froman, Robert C. Tait & L. D. Gottlieb

Authors
  1. Byron E. Froman
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  2. Robert C. Tait
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  3. L. D. Gottlieb
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Communicated by H. Böhme

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Froman, B.E., Tait, R.C. & Gottlieb, L.D. Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli . Mol Gen Genet 217, 126–131 (1989). https://doi.org/10.1007/BF00330951

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  • DOI: https://doi.org/10.1007/BF00330951

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