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Cloning and analysis of two genes for chalcone synthase from Petunia hybrida

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Summary

With the help of a cDNA probe for a chalcone synthase gene of Petroselinum a cDNA clone for a chalcone synthase gene of Petunia hybrida could be identified. The homologous cDNA allowed the cloning of two genomic EcoRI fragments from Petunia hybrida containing complete chalcone synthase genes. It could be demonstrated that the genes on the two fragments are different and are not allelic but members of a gene family. The two genes are found in a variety of different Petunia lines including in the two conditional mutants affected in chalcone synthase expression in floral buds, White Joy and Red Star. The structure of the two chs genes from Petunia is compared to the chs gene from Antirrhinum majus.

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Communicated by P. Starlinger

Dedicated to Professor Georg Melchers to celebrate his 50-year association with the journal

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Reif, H.J., Niesbach, U., Deumling, B. et al. Cloning and analysis of two genes for chalcone synthase from Petunia hybrida . Molec Gen Genet 199, 208–215 (1985). https://doi.org/10.1007/BF00330261

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  • DOI: https://doi.org/10.1007/BF00330261

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