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New data on the karyotype and chromosomal rDNA location in Paradiplozoon megan (Monogenea, Diplozoidae), gill parasite of chubs

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Abstract

New morphological data on chromosome complement of diplozoid parasite Paradiplozoon megan from the chub Squalius cephalus are shown herein. The karyotype of P. megan is characterized by seven pairs (2n = 14) of medium long (up to 11 μm) one-armed chromosomes which are nearly identical in number and morphological classification with chromosomes of other Paradiplozoon species described karyologically to date (Paradiplozoon bliccae, Paradiplozoon nagibinae, Paradiplozoon sapae, Paradiplozoon pavlovskii and Paradiplozoon homoion). A single locus for ribosomal RNA genes, visualized in the secondary constriction site by the fluorescent in situ hybridization method, is situated interstitially on a median part of a long arm of the smallest, 7th chromosome pair in P. megan. Phylogenetic interrelationships within the members of the family Diplozoidae and hypothesis for the ancestral karyotype are discussed here.

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Acknowledgments

Our special thanks are due to Dr. Eva Košková for collecting the material and processing the initial steps of karyological procedures. This work was supported by the grant agency of the Slovak Republic (VEGA 2/0168/13), the project APVV-0653/11 of the Slovak Research and Development Agency and by the Czech Science Foundation (Project No. GAP506/1258). The publication has been created within realization of the project Centre of Excellence for Parasitology (Code ITMS: 26220120022), based on the support of the operational programme “Research & Development” funded from the European Fund of Regional Development (rate 0.2).

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Authors and Affiliations

  1. Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia

    Marta Bombarová & Marta Špakulová

  2. Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic

    Božena Koubková

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  1. Marta Bombarová
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  2. Marta Špakulová
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  3. Božena Koubková
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Correspondence to Marta Bombarová.

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Bombarová, M., Špakulová, M. & Koubková, B. New data on the karyotype and chromosomal rDNA location in Paradiplozoon megan (Monogenea, Diplozoidae), gill parasite of chubs. Parasitol Res 113, 4111–4116 (2014). https://doi.org/10.1007/s00436-014-4082-7

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