Abstract
The DNA replication patterns of the terminal S phase of three species of Mus were analyzed by tritiated thymidine autoradiography. The centromeric heterochromatin of M. fulvidiventris is the latest component to finish DNA synthesis. The Y chromosome finishes replication earlier than the centromeric heterochromatin. The centromeric heterochromatin of M. musculus, on the other hand, is not the latest component to finish DNA synthesis. At the very late S phase, grains are found in the euchromatic arms instead of the heterochromatic areas. The “hot X” and the “hot Y” can be identified in the majority of, but not all, cases. The heterochromatic short arms of the autosomes in M. dunni finish DNA replication earlier than many areas in the euchromatic long arms and the heterochromatin of the sex chromosomes. This indicates that in M. dunni there are at least two types of heterochromatin. The late-replicating zones in the euchromatic long arms are distinctly banded. This banded grain pattern can be seen in all Mus species observed, but in M. dunni it is most exaggerated. Late-replicating chromosome segments can be demonstrated also by 2+ cycles of BUdR incorporation and Giemsa staining.
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Bostock, C. J., Prescott, D. M.: Buoyant density of DNA synthesized at different stages of the S phase of mouse L cells. Exp. Cell Res. 64, 267–274 (1971)
Calderon, D., Schnedl, W.: A comparison between quinacrine fluorescence banding and 3H-thymidine incorporation patterns in human chromosomes. Humangenetik 18, 63–70 (1973)
Church, K.: Replication of chromatin in mouse mammary epithelial cells grown in vitro. Genetics 52, 843–849 (1965)
Craig, A. P., Shaw, M. W.: Autoradiographic studies of the human Y chromosome. Chromosoma (Berl.) 32, 364–377 (1971)
Galton, M., Holt, S. F.: Asynchronous replication in the mouse X chromosome. Exp. Cell Res. 37, 111–116 (1965)
Ganner, E., Evans, H. J.: The relationship between patterns of DNA replication and of quinacrine fluorescence in the human chromosome complement. Chromosoma (Berl.) 35, 326–341 (1971)
Jones, K. W.: Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature (Lond.) 225, 912–915 (1970)
Korenberg, J. R., Freedlender, E. F.: Giemsa technique for the detection of sister chromatid exchanges. Chromosoma (Berl.) 48, 355–360 (1974)
Latt, S. A.: Microfluormetric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)
Markvong, A., Marshall, J. T., Pathak, S., Hsu, T. C.: Chromosomes and DNA of Mus: The karyotypes of M. fulvidiventris and M. dunni. Cytogenet. Cell Genet. 14, 116–125 (1975)
McLaren, A.: Sex-chromosome replication in blood cells of XX/XY Chimeric marmosets. Cytogenet. Cell Genet. 13, 397–402 (1974)
Okada, T. A., Comings, D. E.: Mechanism of chromosome banding III. Similarity between G-bands of mitotic chromosomes and chromomeres of meiotic chromosomes. Chromosoma (Berl.) 48, 65–71 (1974)
Pardue, M. L., Gall, J. G.: Chromosomal localization of mouse satellite DNA. Science 168, 1356–1358 (1970)
Pathak, S., Hsu, T. C., Utakoji, T.: Relationships between patterns of chromosome banding and DNA synthetic sequences: A study on the chromosomes of the Seba's fruit bat, Carollia perspicillata. Cytogenet. Cell Genet. 12, 157–164 (1973)
Perry, P., Wolff, S.: New Giemsa method for the differential staining of sister chromatids. Nature (Lond.) 251, 156–158 (1974)
Sanchez, O., Yunis, J. J.: The relationship between repetitive DNA and chromosomal bands in man. Chromosoma (Berl.) 48, 191–202 (1974)
Stich, H. F., Hsu, T. C.: Cytological identification of male and female somatic cells in the mouse. Exp. Cell Res. 20, 248–250 (1960)
Stubblefield, E.: The kinetics of DNA replication in chromosomes. In: The cell nucleus (H. Busch, ed.), vol. 2, p. 149–162. New York-London: Academic Press 1974
Taylor, J. H., Woods, P. S., Hughes, W. L.: The organization and duplication of chromosomes as revealed by autoradiographic studies using tritium-labeled thymidine. Proc. nat. Acad. Sci. (Wash.) 43, 122–128 (1958)
Wolff, S., Perry, P.: Differential Giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Chromosoma (Berl.) 48, 341–354 (1974)
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Hsu, T.C., Markvong, A. Chromosomes and DNA of Mus: Terminal DNA synthetic sequences in three species. Chromosoma 51, 311–322 (1975). https://doi.org/10.1007/BF00326318
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DOI: https://doi.org/10.1007/BF00326318