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Analysis of meiotic recombination events near a recombination hotspot in the yeast Saccharomyces cerevisiae

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Abstract

The region of yeast chromosome III between the HIS4 and LEU2 genes has an unusually high frequency of meiotic recombination. In order to determine the pattern of cross-over and gene conversion events, we constructed a strain with a number of heterozygous markers in this 25-kb interval. We found that very high levels of reombination are localized to regions of DNA near HIS4. In addition, analysis of the patterns of co-conversion of adjacent markers suggests that there is more than one initiation site contributing to recombination of HIS4.

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Author notes

  1. Michael A. White

    Present address: Cold Spring Harbor Laboratory, P.O. Box, 100, 11724, Cold Spring Harbor, NY, USA

Authors and Affiliations

  1. Department of Biology, University of North Carolina, 27599-3280, Chapel Hill, NC, USA

    Michael A. White & Thomas D. Petes

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  1. Michael A. White
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  2. Thomas D. Petes
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Communicated by M. S. Esposito

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White, M.A., Petes, T.D. Analysis of meiotic recombination events near a recombination hotspot in the yeast Saccharomyces cerevisiae . Curr Genet 26, 21–30 (1994). https://doi.org/10.1007/BF00326300

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