Summary
In systematic toxicological analysis, in which a logical analytical search for a potentially harmful substance has to be carried out, chromatographic methods are of utmost importance since the retention parameter can be used for primary identification. In gas chromatography the Kováts retention index (RI) system is generally accepted for standardization of the retention. In order to increase the reproducibility of the interlaboratory RI, secondary standards for RI calculation have proven to be very useful. The best secondary standards are those that are structurally resembling the substances under investigation. For example, for general toxicological screening analysis, mixtures of drugs are recommended. Stationary phases consisting of Carbowax are most extensively used for solvents and volatile compounds; for less volatile compounds SE-30/OV-1/dimethylsilicone stationary phases have proven to be useful. For these types of stationary phases extensive data compilations comprising over 6000 substances are available for use in toxicology.
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References
Schepers P, Franke JP, De Zeeuw RA (1983) J Anal Toxicol 7:272–278
Kováts E (1958) Helv Chim Acta 41:1915–1932
Blomberg LG (1987) In: Giddings JC, Grushka E, Brown PR (eds) Advances in chromatography, vol 26. Dekker, New York, pp 229–276
Guardino X, Albaiges J, Firpo G, Rodrigues-Vinals R, Gassiot M (1976) J Chromatogr 118:13–22
Van den Dool H, Kratz PD (1963) J Chromatogr 11:463–471
Donike M, Stratmann D (1976) Fresenius Z Anal Chem 279:129–131
Peel HW, Perrigo BJ (1976) Can Soc Forens Sci J 9:69–74
Ardrey RE, De Zeeuw RA, Finkle BS, Franke JP, Moffat AC, Möller MR, Müller RK (1985) Gas-chromatographic retention indices of toxicologically relevant substances on SE-30 or OV-1. VCH, Weinheim
Franke JP, De Zeeuw RA, Wijsbeek J (1987) In: Brandenberger H, Brandenberger R (eds) Reports on forensic toxicology. Proceedings of the 22nd meeting of TIAFT, pp 109–115
Berninger H, Möller MR (1977) Arch Toxicol 37:295–305
Franke JP, Wijsbeek J, De Zeeuw RA (1990) J Forens Sci 35:813–820
Bogusz M, Wijsbeek J, Franke JP, De Zeeuw RA (1983) J High Resol Chromatogr Chromatogr Commun 6:40–42
Bogusz M, Wijsbeek J, Franke JP, De Zeeuw RA (1983) J Anal Toxicol 7:188–192
Bogusz M, Wijsbeek J, Franke JP, De Zeeuw RA, Gierz J (1985) J Anal Toxicol 9:49–54
Yin HF, Zhu Y, Sun YL (1989) Chromatographia 28:502–504
Pacholec F, Poole CF (1984) J Chromatogr 302:289–301
Ballschmiter K, Zell M (1980) Fresenius Z Anal Chem 302:20–31
Donike M (1981) Personal Communication
Watts VW, Simonick TF (1987) J Anal Toxicol 11:210–214
Aderjan R, Bogusz M (1988) J Chromatogr 454:345–351
Rasanen I, Ojanperä I, Vuori E, Hase TA (1992) Proceedings 30th TIAFT Meeting, Fukuoka, Japan (in press)
Vassilaros DL, Kong RC, Later DW, Lee ML (1982) J Chromatogr 252:1–20
Raymer J, Wiesler D, Novotny M (1985) J Chromatogr 325:13–22
Christ DW, Noomano P, Rosas M, Rhone D (1988) J Anal Toxicol 12:84–88
Franke JP, De Zeeuw RA, Wijsbeek J (1988) TIAFT Proceedings of the 25th International Meeting. Groningen, pp 348–355
Moffat AC, Stead AH, Smalldon KW (1974) J Chromatogr 90:19–33
Franke JP, Kuijpers EAP, Machbert G, De Zeeuw RA (1992) In: McLinden V (ed) Proceedings of the 27th International Meeting of TIAFT, Perth, pp 63–70
Parker KD, Fontan CR, Yee JL, Kirk PL (1962) Anal Chem 34:1234–1236
Anthony RM, Suthelmer CA, Sunshine I (1980) J Anal Toxicol 4:43–45
Felby S, Nielsen E (1981) Blutalkohol 18:139–148
Göbel KJ (1982) J Chromatogr 235:119–127
Ramsey JD, Flanagan RJ (1982) J Chromatogr 240:423–444
Christmore DS, Kelly RC, Doshier LA (1984) J Forens Sci 29:1038–1044
Penton Z (1985) Clin Chem 31:439–441
Franke JP, Wijsbeek J, De Zeeuw RA, Möller MR, Niermeyer H (1988) J Anal Toxicol 12:20–24
Pfleger K, Maurer HH, Weber A (1985) Mass spectral and GC data of drugs, poisons and their metabolites. VCH, Weinheim
Mills III T, Roberson JC (1987) Instrumental data for drug analysis. Elsevier, New York, Amsterdam, London
Japp M, Gill R, Osselton MD, Cordonnier J (1986) In: Heyndrickx B (ed) Proceedings of the 23rd European International Meeting of TIAFT, Ghent, pp 172–190
De Zeeuw RA, Franke JP, Geldmacher-von Mallinckrodt M, Machata G, Möller MR, Müller RK, Gräfe A, Tiess D (1992) Gas chromatographic retention indices of solvents and volatile substances for use in toxicological analysis. VCH, Weinheim
Tiess D (1984) Wiss Z Wilhelm-Pieck Univ Rostock 33:6–9
Streete PJ, Ruprah M, Ramsey JD, Flanagan RJ (1992) Analyst 117:1111–1127
Huizer H (1991) In: Gough TA (ed) The analysis of drugs of abuse. Wiley, Chichester, pp 23–92
Japp M, Gill R, Osselton MD (1987) J Forens Sci 32:1574–1586
De Zeeuw RA, Franke JP, Maurer HH, Pfleger K (1992) Gas chromatographic retention indices of toxicologically relevant substances on packed or capillary columns with dimethylsilicone stationary phases. VCH, Weinheim
Daldrup T, Susanto F, Michalke P (1981) Fresenius Z Anal Chem 308:413–427
Post D (1983) Schnelle gas-chromatographische Arzneimittelerkennung. Hüthig, Heidelberg
Perrigo BJ, Peel HW (1981) J Chromatogr Sci 19:219–226
Manca D, Ferron L, Weber J-P (1989) Clin Chem 35:601–607
Moffat AC (1986) Clarke's isolation and identification of drugs. Pharmaceutical Press, London
Perrigo BJ, Peel HW, Ballantyne DJ (1985) J Chromatogr 341:81–88
Franke JP, Hegge HJG, De Zeeuw RA (1992) Merck toxicology screening system. Merck, Darmstadt
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Franke, J.P., Bogusz, M. & de Zeeuw, R.A. An overview on the standardization of chromatographic methods for screening analysis in toxicology by means of retention indices and secondary standards. Fresenius J Anal Chem 347, 67–72 (1993). https://doi.org/10.1007/BF00322833
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DOI: https://doi.org/10.1007/BF00322833