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Speciation and simultaneous determination of mercury species in dolphin liver by liquid chromatography with on-line cold vapor atomic absorption spectrometry

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Summary

An interface for coupling liquid chromatography with atomic absorption spectrometry is described and fully evaluated. Inorganic, methyl and ethyl mercury are separated as cysteinato complexes by isocratic elution on a reversed-phase column. Protein bound mercury is released from dolphin liver homogenate by acid hydrolysis. Inorganic and methyl mercury are simultaneously determined by direct on-column injection of liver hydrolysate previously subjected to a simple centrifugation step. No sample clean-up, solvent extraction or chemical derivatization step is required. Detection limits are in the order of 200 ng/g of tissue. The method is highly selective for Hg species: no spurious peak was observed even with complex samples such as liver hydrolysate. A sampling rate of 12 samples/h was achieved.

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Authors and Affiliations

  1. Laboratorio di Chimica Analitica, Dipartimento di Chimica dell'Universita Trav., 200 Re David, I-70126, Bari, Italy

    F. Palmisano & P. G. Zambonin

  2. Istituto Sperimentale Talassografico, Consiglio Nazionale delle Ricerche, Via Roma 3, I-74100, Taranto, Italy

    N. Cardellicchio

Authors
  1. F. Palmisano
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  2. P. G. Zambonin
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  3. N. Cardellicchio
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Dedicated to Professor Dr. Wilhelm Fresenius on the occasion of his 80th birthday

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Palmisano, F., Zambonin, P.G. & Cardellicchio, N. Speciation and simultaneous determination of mercury species in dolphin liver by liquid chromatography with on-line cold vapor atomic absorption spectrometry. Fresenius J Anal Chem 346, 648–652 (1993). https://doi.org/10.1007/BF00321264

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  • DOI: https://doi.org/10.1007/BF00321264

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