Summary
There are at least three alcohol dehydrogenases in Aspergillus nidulans. ADHII has been observed in polyacrylamide gels stained for ADH activity but, unlike ADHI and ADHIII, no physiological function has been attributed to it. This paper describes mutations that have been isolated from strains carrying a deletion in the structural gene for ADHI (alcA) and its adjacent positively-acting regulatory gene (alcR) that restore some ability to utilise ethanol as a carbon source. The mutations map at three loci, and all show elevated levels of the ADHII staining band. An assay for ADHII has been developed. The growth on ethanol has been shown to be dependent on the previously identified aldehyde dehydrogenase (structural gene, aldA). Two of the mutations, alcD and alcE, represent newly discovered mutations affecting ethanol utilisation while the third mutation is in amdA, a previously described trans-acting regulatory protein.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Atkinson PW, King JA, Hynes MJ (1985) Curr Genet 10:133–138
Clutterbuck AJ (1974) In: King RC (ed) Handbook of genetics. Plenumm Press, New York, pp 447–510
Clutterbuck AJ (1987) In: Genetics maps. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 325–335
Cove DJ (1966) Biochim Biophys Acta 113:51–56
Creaser EH, Porter RL, Britt KA, Pateman JA, Doy CH (1984) Biochem J 225:449–454
Goa J (1953) Scand J Clin Lab Invest 5:218–222
Herbert D, Phipps PJ, Strange RE (1971) In: Norris JR, Ribbons DW (eds) Methods in microbiology 5B. Academic Press, New York, pp 210–344
Hynes MJ (1978) Mol Gen Genet 161:59–65
Jones IG, Sealy-Lewis HM (1989) Curr Genet 15:135–142
Jones IG, Sealy-Lewis HM (1990) Curr Genet, 17:81–83
Laemmli UK (1970) Nature (London) 277:680–685
Lockington RA, Sealy-Lewis HM, Scazzocchio C, Davies RW (1985) Gene 33:137–149
McKnight GL, Kato H, Upshall A, Parker MD, Saari G, O'Hara PJ (1985) EMBO J 4:2093–2099
Oakley CE, Weil CF, Kretz PL, Oakley BR (1987) Gene 53:293–298
O'Connell MJ, Kelly JM (1988) Curr Genet 14(2):95–104
Patemen JA, Doy CH, Olsen JE, Norris V, Creaser EH, Hynes MJ (1983) Proc R Soc London Ser B 217:243–264
Pickett M, Gwynne DI, Buxton FP, Elliott R, Davies RW, Lockington RA, Scazocchio C, Sealy-Lewis HM (1987) Gene 51:217–222
Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Bufton AWJ (1953) Adv Genet 5:141–238
Rowland LJ, Strommer JN (1986) Mol Cell Biol 6:3368–3372
Sealy-Lewis HM, Lockington RA (1984) Curr Genet 8:253–259
Author information
Authors and Affiliations
Additional information
Communicated by B. S. Cox
Rights and permissions
About this article
Cite this article
Sealy-Lewis, H.M. The identification of mutations in Aspergillus nidulans that lead to increased levels of ADHII. Curr Genet 18, 65–70 (1990). https://doi.org/10.1007/BF00321117
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00321117