Summary
About one-half of the ribosomal repeat unit of two isolates of Pythium ultimum was amplified by means of the polymerase chain reaction using one primer pair. The amplified region includes a small part of the large subunit ribosomal RNA gene, about half of the small subunit ribosomal RNA gene, and the entire intergenic region. The intergenic region of both isolates of P. ultimum has length heterogeneity due to the presence of subrepeat arrays (Klassen and Buchko 1990). PCR amplification of the heterogeneous target DNA resulted in sets of fragments which accurately reflect the heterogeneity in the target DNA, although there is a preferential amplification of the smaller targets. PCR product sizes ranged from 4.6 to 5.8 kb.
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Barr DJS (1983) In: Buczacki SJ (ed) Zoosporic plant pathogens —a modern prospective. Academic Press, New York, pp 43–83
Cluster PD, Marinkovic D, Allard RW, Ayala FJ (1987) Proc Natl Acad Sci USA 84:610–614
Dams E, Hendriks L, Van de Peer Y, Neefs J-M, Smits G, Vandenbempt I, De Wachter R (1988) Nucleic Acids Res 16:187–173
Flavell RB, O'Dell M, Sharp P, Nevo E, Beiles A (1986) Mol Biol Evol 3:547–558
Free SJ, Rice PW, Metzenberg RL (1979) J Bacteriol 137:1219–1226
Gutell RR, Fox GE (1988) Nucleic Acids Res 16:175–269
Jeffreys AJ, Wilson V, Keyte J (1988) Nucleic Acids Res 16:10953–10971
Klassen GR, Buchko J (1990) Curr Genet 17:125–127
Klassen GR, McNabb SA, Dick MW (1987) J Gen Microbiol 133:2953–2959
Mullis K, Faloona F, Scharf S, Saiki RK, Horn G, Erlich H (1986) Cold Spring Harbor Symp Quant Biol 51:263–273
Reeder RH (1984) Cell 38:349–351
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Science 239:487–491
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Communicated by C. W. Birky
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Buchko, J., Klassen, G.R. Detection of length heterogeneity in the ribosomal DNA of Pythium ultimum by PCR amplification of the intergenic region. Curr Genet 18, 203–205 (1990). https://doi.org/10.1007/BF00318381
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DOI: https://doi.org/10.1007/BF00318381