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RNA editing of sorghum mitochondrial atp6 transcripts changes 15 amino acids and generates a carboxy-terminus identical to yeast

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Summary

Sequencing of sorghum mitochondrial atp6 cDNA clones revealed 19 C-to-U transcript editing events within a 756 bp-conserved core gene; three were silent and 16 resulted in 15 amino acid changes. Only one edit, which was silent, was found in the 381 bp amino-extension to the core gene. Eleven of the 15 changed amino acids were identical with or else represented conservative changes compared to yeast atp6. Editing of a CAA codon to TAA truncates to carboxy-terminus to a position identical to that of yeast. The frequency of editing at sites which change amino acids was very high in contrast to partial editing at silent, third base, sites.

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References

  • Begu D, Graves PV, Domec C, Arselin G, Litvak S, Araya A (1990) The Plant Cell 21:1283–1290

    Google Scholar 

  • Bland MM, Levings CS III, Matzinger DF (1987) Curr Genet 12:475–481

    Google Scholar 

  • Bonen L, Bird S (1988) Gene 73:47–56

    Google Scholar 

  • Chen SH, Habib G, Yang CY, Gu ZB, Lee BR, Weng SA, Silberman SR, Cai SJ, Deslypere JP, Rossenen M, Gotto AM, Li WH, Chan L (1987) Science 238:363–366

    Google Scholar 

  • Covello PS, Gray MW (1989) Nature 341:662–666

    Google Scholar 

  • Covello PS, Gray MW (1990) Nucleic Acids Res 18:5189–5196

    Google Scholar 

  • Dewey RE, Levings CS III, Timothy DH (1985) Plant Phys 79:914–919

    Google Scholar 

  • Feagin JE, Abraham JM, Stuart K, (1989) Cell 53:413–422

    Google Scholar 

  • Fragoso LL, Nichols SE, Levings CS III (1989) Genome 31:160–168

    Google Scholar 

  • Grabau E, Havlik M, Gesteland R (1988) Curr Genet 13:83–89

    Google Scholar 

  • Graves PV, Begu J, Velours J, Neau E, Belloc F, Litvak S, Araya A (1990) J Mol Biol 214:1–6

    Google Scholar 

  • Gualberto JM, Lamattina L, Bonnard G, Weil JH, Grienberger JM (1989) Nature 341:660–662

    Google Scholar 

  • Gualberto JM, Weil JH, Grienenberger JM (1990) Nucleic Acids Res 18:3771–3776

    Google Scholar 

  • Hiesel R, Wissinger B, Schuster W, Brennicke A (1989) Science 246:1632–1634

    Google Scholar 

  • Hiesel R, Wissinger B, Brennicke A (1990) Curr Genet 18:371–375

    Google Scholar 

  • Kadowaki K, Suzuki T, Kazama S (1990) Mol Gen Genet 224:10–16

    Google Scholar 

  • Kawasaki ES, Wang AM (1989) In: Erlich H (ed) PCR Technology, principles and applications for DNA amplification. Stockton Press, New York, pp 89–97

    Google Scholar 

  • Kennell JC, Pring DR (1989) Mol Gen Genet 216:16–24

    Google Scholar 

  • Macfarlane JL, Wahleithner JA, Wostenholme DR (1990) Curr Genet 18:87–91

    Google Scholar 

  • Macino G, Tzagoloff A (1980) Cell 20:507–517

    Google Scholar 

  • Makaroff CA, Apel IJ, Palmer JD (1989) J Biol Chem 264:11706–11713

    Google Scholar 

  • Michon T, Galante M, Velours J (1988) Eur J Biochem 172:621–625

    Google Scholar 

  • Nowak C, Kück U (1990) Nucleic Acids Res 18:7164

    Google Scholar 

  • Powell LM, Wallis SC, Pease RJ, Edwards YH, Knott TJ, Scott J (1987) Cell 50:831–840

    Google Scholar 

  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Science 239:487–491

    Google Scholar 

  • Schuster W, Brennicke A (1987) Nucleic Acids Res 15:9092

    Google Scholar 

  • Schuster W, Brennicke A (1990) FEBS Lett 268:252–256

    Google Scholar 

  • Schuster W, Hiesel R, Wissinger B, Brennicke A (1990a) Mol Cell Biol 10:2428–2431

    Google Scholar 

  • Schuster W, Unseld M, Wissinger B, Brennicke A (1990b) Nucleic Acids Res 18:229–233

    Google Scholar 

  • Schuster W, Wissinger B, Unseld M, Brennicke A (1990c) EMBO J 9:263–269

    Google Scholar 

  • Walker JE, Saraste M, Gay NJ (1984) Biochim Biophys Acta 768:164–200

    Google Scholar 

  • Weissmann C, Cattaneo R, Billeter MA (1990) Nature 343:697–699

    Google Scholar 

  • Wintz H, Hanson MR (1990) Curr Genet 19:61–64

    Google Scholar 

  • Wissinger B, Schuster W, Brennicke A (1990) Mol Gen Genet 224:389–395

    Google Scholar 

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Author notes

  1. F. Kempken

    Present address: Department of Botany, Ruhr-University, P.O. Box 102148, W-4630, Bochum

  2. J. A. Mullen

    Present address: Department of Biological Sciences, Northern Illinois University, 60115, Dekalb, IL, USA

Authors and Affiliations

  1. Department of Plant Pathology and USDA-ARS, University of Florida, 32611, Gainesville, FL, USA

    F. Kempken, J. A. Mullen, D. R. Pring & H. V. Tang

Authors
  1. F. Kempken
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  2. J. A. Mullen
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  3. D. R. Pring
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  4. H. V. Tang
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Communicated by K. Esser

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Kempken, F., Mullen, J.A., Pring, D.R. et al. RNA editing of sorghum mitochondrial atp6 transcripts changes 15 amino acids and generates a carboxy-terminus identical to yeast. Curr Genet 20, 417–422 (1991). https://doi.org/10.1007/BF00317071

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