Abstract
The effect of soman on rat brain ribosomes organization and translational activity of mRNA in cellfree system was studied in rats exposed to 1.3 LD50 soman (120 μg/kg body weight) and in rats repeatedly injected with 0.4 LD50 soman (35 μg/kg). Fifteen minutes after the injection of 1.3 LD50 soman the heavy polyribosomal fraction from rat brain was found to be enriched and translational activity of mRNA was enhanced. In rats administered five injections of 0.4 LD50 soman at 24-h intervals, the low density ribosomes appeared as the predominant fraction whereas the activity of mRNA in all cell-free system was significantly impaired. It is concluded that soman intoxication expresses a stimulatory or inhibitory effect on the processes of protein synthesis in the rat brain, depending on the dose schedule of soman administration.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anthony A, Doebler JA, Bocan TMA, Shih T-M (1983) Scanningintegrating microdensitometric analyses of brain neuronal RNA and acetylcholinesterase in acute soman treated rats. Cell Biochem Funct 1: 30–36
Chirgwin JM, Przybila AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299
Doebler JA, Bocan TMA, Moore RA, Shih T-M, Anthony A (1983) Brain neuronal RNA metabolism during acute soman toxication: effects of antidotal pretreatments. Neurochem Res 8: 997–1011
Doebler JA, Moore RA, Wall TJ, Anthony A (1984a) Brain neuronal RNA metabolism during sustained low-level soman toxication. Life Sci 34: 659–667
Doebler JA, Wall TJ, Moore RA, Martin LJ, Shih T-M, Anthony A (1984b) Cytophotometric analyses of brain neuronal RNA in soman intoxicated rabbits. Toxicology 32: 153–163
Doebler JA, Martin LJ, Shih T-M, Anthony A (1985) Cytophotometric analyses of mesencephalic reticular formation neuronal RNA in soman intoxicated rats. Neurochem Int 7(3): 415–420
Drewes LR, Singh AK (1985) Transport, metabolism and blood flow in brain during OP induced seizures. Proc West Pharm Soc 28: 191–195
Glišin V, Crkvenjakov R, Byus C (1974) Ribonucleid acid isolated by cesium chloride centrifugation. Biochemistry 13: 2633–2637
Gilbert JM, Strocchi P, Brown BA, Marotta CA (1981) Tubulin synthesis in rat forebrain: studies with free and membranebound polysomes. J Neurochem 36(3): 839–846
Held W, West K, Gallagher J (1977) Importance of initiation factor preparation in the translation of reovirus and globin mRNA lacking a 5′-terminal 7-methylguanosine. J Biol Chem 252: 8489–8495
Jović R, Milošević M (1970) Effective doses of some cholinolytics in the treatment of anticholinesterase poisoning. Eur J Pharmacol 12: 85–93
Mahler HR, Brown BJ (1968) Protein synthesis by cerebral cortex polysomes: characterization of the system. Arch Biochem Biophys 125: 387–400
Marotta CA, Strocchi P, Gilbert JM (1979) Biosynthesis of heterogenous forms of mammalian brain tubulin subunits by multiple messenger RNAs. J Neurochem 33: 231–346
Martin LJ, Doebler JA, Anthony A (1986a) Perykarial RNA changes within neurons of the candate-putamen and substantia nigra in soman intoxicated rats. Neurochem Int 9(1): 121–126
Martin LJ, Doebler JA, Wall TJ, Shih T-M, Anthony A (1986b) Brain neuronal chromatin responses in acute soman intoxicated rats. Neurochem Res 11(8): 1203–1215
McLeod CG, Singer AW, Harrington DG (1984) Acute neuropathology in soman poisoned rats. Neurotoxicology 5(2): 53–58
Murthy MRV (1972) Free and membrane-bound ribosomes of rat erebral cortex. J Biol Chem 25: 1936–1943
O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250(10): 4007–4021
Pazdernik TL, Cross RS, Nelson SR, Samson FE, McDonough JH (1983) Soman-induced depression of brain activity of TAB-pretreated rats: 2-deoxyglucose study. J Neurotoxicol 4: 27–34
Pazdernik TL, Cross RS, Giesler M, Samson FE, Nelson SR (1985) Changes in local cerebral glucose utilization induced by convulsants. Neuroscience 14(3): 823–835
Roberts S, Morelos BS (1980) Cerebral ribosomal protein phosphorylation in experimental hyperphenylalaninaemia. Biochem J 190: 405–419
Samson FE, Pazdernik TL, Cross RS, Giesler MP, Mewes K, Nelson SR, McDonough JH (1984) Soman induced changes in regional glucose use. Fund Appl Toxicol 4: 173–183
Sicora-VanMeter KC, Wierwille RC, Willetts EJ, VanMeter WG (1987) Morphological evidence for increased protein synthesis in CNS neurons after soman exposure. Fund Appl Toxicol 8: 23–28
Sokoloff L (1981) Localization of functional activity in the central nervous system by measurement of glucose utilization with radioactive deoxyglucose. J Cerebral Blood Flow Metab 1: 7–36
Stitcher DL, Harris LW, Moore RD, Heyl WC (1977) Synthesis of cholinesterase following poisoning with irreversible anticholinesterases: effects of theophylline and N6,2-dibutyryl adenosine 3′,5′-monophosphate on synthesis and survival. Toxicol Appl Pharmacol 41: 79–90
Sevaljević L, Brajanović N, Krtolica K, Tomić M, Bosković B (1986) The effect of soman poisoning on the rate of RNA synthesis in vivo and in isolated nuclei. J Toxicol Clin Exp 6(5): 297–305
Viana GB, Kauffman FC (1983) Effects of soman on energy state and selected metabolites in mouse brain. Soc Neurosci Abstr 9: 210–219
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ševaljević, L., Bošković, B., Glibetić, M. et al. Effect of soman intoxication on the organization of rat brain ribosomes and the translational activity of mRNA in a cell-free system. Arch Toxicol 63, 244–247 (1989). https://doi.org/10.1007/BF00316376
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00316376