Summary
In Saccharmmyces cerevisiae, the COR2 gene codes for the 40 kDa subunit II of the QH2: cytochrome c oxidoreductase, an enzyme of the mitochondrial respiratory chain. Regions in the 5′ flank of this gene important for regulated expression were identified by assaying β-galactosidase activities in cells carrying different COR2-lacZ fusion genes. Sequences downstream of position-201 relative to the translational initiation codon are sufficient to confer regulation by carbon source, whereas sequences downstream of position-153 do not give rise to significant expression. A binding site for the abundant general transcription factor GFI is present in the region between-201 and-153 just upstream from sequences which resemble the consensus DNA recognition sequence of the regulatory protein complex HAP2/HAP3: 5′-TNATTGGT-3′. By quantitating RNA levels and assaying β-galactosidase activities we show that synthesis of COR2, which is not a hemoprotein, is regulated by HAP1, HAP2/HAP3 and heme.
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References
Arcangioli B, Lescure B (1985) EMBO J 4:2627–2633
Bradford MM (1976) Anal Biochem 72:248–254
Dente L, Cesareni G, Cortese R (1983) Nucleic Acids Res 11:1645–1655
De Vries S, Marres CAM (1988) Biochim Biophys Acta 895:205–239
Dorsman JC, van Heeswijk WC, Grivell LA (1988) Nucleic Acids Res 16:7287–7301
Dorsman JC, Doorenbosch MM, Maurer CTC, de Winde JH, Mager WH, Planta RJ, Grivell LA (1989) Nucleic Acids Res 17:4917–4923
Forsburg SL, Guarente L (1988) Mol Cell Biol 8:647–654
Guarente L, Ptashne M (1981) Proc Natl Acad Sci USA 78:2199–2203
Guarente L, Lalonde B, Gifford P, Alani E (1984) Cell 36:503–511
Guarente L (1987) Annu Rev Genet 21:425–452
Grivell LA (1989) Eur J Biochem 182:477–493
Hamil KG, Nam HG, Fried HM (1988) Mol Cell Biol 8:4328–4341
Klebe RJ, Harriss JV, Sharp ZD, Douglas MG (1983) Gene 25:333–341
Lowry CV, Zitomer RS (1984) Proc Natl Acad Sci USA 81:6129–6133
Lowry CV, Lieber RH (1986) Mol Cell Biol 6:4145–4148
Maarse AC, De Haan M, Bout A, Grivell LA (1988a) Nucleic Acids Res 16:5797–5811
Maarse AC, De Haan M, Schoppink PJ, Berden JA, Grivell LA (1988b) Eur J Biochem 172:179–184
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Mattoon JR, Lancashire WE, Sanders HK, Carvajal E, Malamud D, Brag GRC, Panek AD (1979) In: Caughey WJ (ed) Biochemical and clinical aspects of oxygen. Academic Press, New York, pp 421–435
Messing J (1983) In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology, 101, Academic Press, New York, pp 20–78
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Olesen J, Hahn S, Guarente L (1987) Cell 51:953–961
Oudshoorn P, van Steeg H, Swinkels B, Schoppink P, Grivell LA (1987) Eur J Biochem 163:97–103
Panzeri L, Philippsen P (1982) EMBO J 1:1605–1611
Pfeifer K, Arcangioli B, Guarente L (1987a) Cell 49:9–18
Pfeifer K, Prezant T, Guarente L (1987b) Cell 49:19–27
Pruijn GJM, Van Miltenburg RT, Claessens JAJ, Van der Vliet PC (1988) J Virol 62:3092–3102
Quain DE, Haslam JM (1979) J Gen Microbiol 111:343–351
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sherman F, Fink GR, Hicks JB (1986) Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sousa R, Arcangioli B (1989) EMBO J 8:1801–1808
Thorsness M, Schafer W, D'Ari L, Rine J (1989) Mol Cell Biol 9:5702–5712
Trawick JD, Wright RM, Poyton RO (1989) J Biol Chem 264:7005–7008
Trueblood CE, Wright RM, Poyton RO (1988) Mol Cell Biol 8:4537–4540
Winkler H, Adam G, Mattes E, Schanz M, Hartig A, Ruis H (1988) EMBO J 7:1799–1804
Wright RM, Trawick JD, Trueblood CE, Patterson TE, Poyton RO (1987) In: Papa S, Chance B, Ernster L (eds) Cytochrome systems: molecular biology and bioenergetics, Plenum Press, New York, pp 49–56
Zitomer RS, Montgomery DL, Nichols DL, Hall BD (1979) Proc Nalt Acad Sci USA 76:3627–3631
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Dorsman, J.C., Grivell, L.A. Expression of the gene encoding subunit II of yeast QH2: Cytochrome c oxidoreductase is regulated by multiple factors. Curr Genet 17, 459–464 (1990). https://doi.org/10.1007/BF00313072
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DOI: https://doi.org/10.1007/BF00313072