Summary
Using the previously cloned Aspergillus niger N756 pectin lyase D gene as a probe, the corresponding pelD gene has been isolated from a genomic library of the loboratory strain A. niger N400. This gene encodes PLD, previously described as PLI, which is one of the two major pectin lyases isolated from the commeriial pectinase preparation Ultrazym®. Heterologous hybridization of the A. niger N400 genomic library with the pelD gene led to the isolation of another five genes: pelA, B, C, E, and F. These genes differ in their hybridization patterns with probes containing either the entire pelD gene, or 5′ or 3′ parts thereof. By partial sequencing, and expression in an A. niger transformant containing multiple copies of the pelA gene, we show that this gene, which hybridizes strongest with the pelD gene, encodes the other major pectin lyase from Ultrazym®, PLII.
Similar content being viewed by others
References
BeMiller, JN (1986) In: Fisherman ML, Jen JJ (eds) Chemistry and function of pectins. American Chemical Society, Washington, DC
Benton WD, Davis RW (1977) Science 196:180–182
Dean RA, Timberlake WE (1989) The Plant Cell 1:275–284
de Vries JA, Voragen AGJ, Rombouts FM, Pilnik W (1986) In: Fisherman ML, Jen JJ (eds) Chemistry and function of pectins. American Chemical Society, Washington DC
Edman P, Begg G (1967) Eur J Biochem 1:80–91
Frank G, Strubert W (1973) Chromatographia 6:522–524
Frischauf AM, Lehrach H, Poustra A, Murray N (1983) J Mol Biol 170:827–842
Gines MJ, Dove MJ, Seligy VL (1989) Gene 79:107–117
Goddard JM, Caput D, Williams SR, Martin DW (1983) Proc Natl Acad Sci USA 80:4281–4285
Goosen T, Bloemheuvel G, Gysler C, de Bie DA, van de Broek HWJ, Swart K (1987) Curr Genet 11:499–503
Gwynne DI, Buxton FP, Sibley S, Davies RW, Lockington RA, Scazzocchio C, Sealy-Lewis HM (1987) Gene 51:205–216
Gysler C, Harmsen JAM, Kester HCM, Visser J, Heim J (1990) Gene, in press
Maniatis T, Fritsch EF, Sambrook J (1992) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
May GS, Tsang M-S, Smith H, Fidel S, Morris NR (1987) Gene 55:231–243
McNeil M, Darvill AG, Fry SC, Albersheim P (1984) Annu Rev Biochem 53:625–636
Messing J (1983) Methods Enzymol 101C:20–78
Pettila M, Lehtovaara P, Nevalainen H, Bhikhabhai R, Knowles J (1986) Gene 45:253–263
Pilnik W (1982) in: P. Dupuy (ed) Use of enzymes in the beverage industry. Technique et documentation. Lavoisier, Paris
Pontecorvo G, Roper JA, Hemmons LJ, MacDonald KD, Bufton AWJ (1953) Adv Genet 5:141–238
Sanger F, Nickelen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sundstrom P, Lira LM, Choi D, Linz JE, Syphers PS (1987) Nucleic Acid Res 15:9997–10006
van Houdenhoven FEA (1975) Studies on pectin lyase. Thesis Agricultural University Wageningen, The Netherlands
von Heijne G (1985) J Mol Biol 184:99–105
Wirsel S, Lachmund A, Wildhardt G, Ruttkowski E (1989) Mol Microbiol 3:3–14
Yelton MM, Hamer JE, Timberlake WE (1984) Proc Natl Acad Sci USA 81:1470–1474
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harmsen, J.A.M., Kusters-van Someren, M.A. & Visser, J. Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): Indications of a pectin lyase gene family in A. niger . Curr Genet 18, 161–166 (1990). https://doi.org/10.1007/BF00312604
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00312604