Abstract
We have developed rapid and economic methods for the isolation of nucleic acids from filamentous fungi. The main advantages of these methods are: (1) the mycelium is directly recovered from a Petri-dish culture, (2) the complete experiment takes place in microfuge tubes, (3) it is very fast and allows for the processing of 24 samples in the same day, and (4) up to 100 μg of total DNA or RNA are recovered, both of which are sufficiently pure for most purposes. Of particular interest is the recovery of large amounts of mitochondrial DNA as visualised by electrophoresis in ethidium bromidestained gels.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cummings DJ, Belcour L, Grandchamp C (1979) Mol Gen Genet 171:229–238
DuTeau NM, Leslie JF (1991) Fungal Genet Newslett 38:72
Möller EM, Bahnweg G, Sandermann H, Geiger HH (1992) Nucleic Acids Res 20:6115–6116
Osiewacz HD, Esser K (1983) Curr Genet 7:219–223
Picard M, Debuchy R Coppin E (1991) Genetics 128:539–547
Silar P, Wegnez M (1990) FEBS Lett 269:273–276
Author information
Authors and Affiliations
Additional information
Communicated by O. C. Yoder
Rights and permissions
About this article
Cite this article
Lecellier, G., Silar, P. Rapid methods for nucleic acids extraction from Petri dish-grown mycelia. Curr Genet 25, 122–123 (1994). https://doi.org/10.1007/BF00309536
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00309536