Summary
Intraventricular injections of moderate doses (25–75μg) of 5,7-dihydroxytryptamine (5,7-DHT) into the left lateral ventricle of ether anaesthetized rats cause pronounced damage to CNS indoleamine axons, reflected by accumulations of large amounts of serotonin in distorted, heavily swollen axons, so called indoleamine droplet fibres. Larger doses (100, 150 or 300 μg) provoke a piling up of catecholamines in drug affected preterminal catecholamine containing fibres besides extensive lesioning of indoleamine axons.
5,7-DHT condenses with formaldehyde to form a light yellow fluorescent compound. Uptake and accumulation of 5,7-DHT into indoleamine terminals and axons—as revealed in short term experiments—provides a means of mapping of indoleamine neurons in the rat brain.
Following the application of 5,7-DHT (25–150 μg), rats develop characteristic behavioural disturbances, as e.g. increased sensitivity to sensory stimulation, and a failure to habituate to repeatedly applied sensory stimuli, and bizarre social behaviour, i.e. repeated fighting attacks in an unusual upright posture. These alterations resemble those observed after 5,6-DHT and may be indicative of a deprivation of the brain from functional serotonin.
5,7-DHT is considered to be an important, additional tool for the investigation of serotonin neurons and problems of serotonin transmission in the mammalian brain.
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Baumgarten, H. G.: Uptake and distribution of false transmitters and their precursors in adrenergic sympathetic nerves of the guinea-pig vas deferens. In: Morphological aspects of andrology (eds. Holstein, A. F., Horstmann, E.), p. 141–146. Fortschr. Androl., vol. I. Berlin: Grosse 1970.
Baumgarten, H. G.: Biogenic monoamines in the cyclostome and lower vertebrate brain. Progr. Histochem. Cytochem. 4, No 1 (1972).
Baumgarten, H. G., Björklund, A., Holstein, A. F., Nobin, A.: Chemical degeneration of indolamine axons in rat brain by 5,6-dihydroxytryptamine. Z. Zellforsch. 129, 256–271 (1972).
Baumgarten, H. G., Björklund, A., Lachenmayer, L., Nobin, A., Stenevi, U.: Long-lasting selective depletion of brain serotonin by 5,6-dihydroxytryptamine. Acta physiol. scand., Suppl. 373 (1971).
Baumgarten, H. G., Björklund, A., Lachenmayer, L., Nobin, A., Stenevi, U.: Studies on the mechanism of action of 5,6-dihydroxytryptamine on the bulbospinal serotonin neuron system. II. Fluorescence microscopical and chemical evidence for a selective degeneration of serotonin axons. To be published (1972).
Baumgarten, H. G., Evetts, K. D., Holman, R. B., Iversen, L. L., Vogt, M., Wilson, G.: Effects of 5,6-dihydroxytryptamine on monoaminergic neurones in the central nervous system of the rat. J. Neurochem. 19, 1587–1597 (1972).
Baumgarten, H. G., Göthert, M., Holstein, A. F., Schlossberger, H. G.: Chemical sympathectomy induced by 5,6-dihydroxytryptamine. Z. Zellforsch. 128, 115–134 (1972).
Baumgarten, H. G., Lachenmayer, L.: Chemically induced degeneration of indoleamine-containing nerve terminals in rat brain. Brain Res. 38, 228–232 (1972).
Baumgarten, H. G., Lachenmayer, L.: Selektive, chemisch induzierte Degeneration monoaminerger Neuronensysteme im Säugergehirn: eine neue Methode für die experimentelle Neuroanatomie und Neuropharmakologie. Dtsch. med. Wschr., in press (1972b).
Baumgarten, H. G., Lachenmayer, L., Schlossberger, H. G.: Evidence for a degeneration of indoleamine containing nerve terminals in rat brain, induced by 5,6-dihydroxytryptamine. Z. Zellforsch. 125, 553–569 (1972).
Baumgarten, H. G., Schlossberger, H. G.: Effects of 5,6-dihydroxytryptamine on brain monoamine neurons in the rat. In: Serotonin and behavior. Academic Press 1972 in press.
Baumgarten, H. G., Victor, S. J., Lovenberg, W.: The effect of intraventricular 5,6-dihydroxytryptamine on tryptophan hydroxylase in various brain regions of the rat. In preparation (1972).
Björklund, A., Katzman, R., Stenevi, U., West, K. A.: Development and growth of axonal sprouts from noradrenaline and 5-hydroxytryptamine neurons in the rat spinal cord. Brain. Res. 31, 21–33 (1971).
Dahlström, A., Fuxe, K.: Evidence for the existence of monoamine containing neurons in the central nervous system. I. Demonstration of monoamines in the cell bodies of brain stem neurons. Acta physiol. scand 62, Suppl. 232 (1964). II. Experimentally induced changes in the intraneuronal amine levels of bulbospinal neuron systems. Acta physiol. scand. 64, Suppl. 247 (1965).
Falck, B., Owman, Ch.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. lund. 2, No 7, 1–23 (1965).
Fuxe, K., Ungerstedt, U.: Histochemical studies on the distribution of catecholamines and 5-hydroxytryptamine after intraventricular injections. Histochemie 13, 16–28 (1968).
Korf, J., Kuiper, H. E.: Induction of bizarre behaviour in rats by p-chloroamphetamine, a serotonin depletor, after repeated drug administration. Psychopharmacologia (Berl.) 21, 328–337 (1971).
Noble, E. P., Wurtman, R. J., Axelrod, J.: A simple and rapid method for injecting H3-norepinephrine into the lateral ventricle of the rat brain. Life Sci. 6, 281–291 (1967).
Rossum, J. M. van: Mode of action of psycho-motor stimulant drugs. Int. Rev. Neurobiol. 12, 307–383 (1970).
Shaskan, E. G., Snyder, S. H.: Kinetics of serotonin accumulation into slices from rat brain: Relationship to catecholamine uptake. J. Pharmacol. exp. Ther. 175, 404–418 (1970).
Ungerstedt, U.: I. Stereotaxic mapping of the monoamines pathways in the rat brain. Acta physiol. scand., Suppl. 367 (1971).
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Dedicated to Prof. Dr. Dr. R. Janzen with the best wishes for his 65th birthday.
Supported by the Deutsche Forschungsgemeinschaft.
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Baumgarten, H.G., Lachenmayer, L. 5,7-Dihydroxytryptamine: Improvement in chemical lesioning of indoleamine neurons in the mammalian brain. Z.Zellforsch 135, 399–414 (1972). https://doi.org/10.1007/BF00307184
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DOI: https://doi.org/10.1007/BF00307184