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Erhöhte Rekombinationshäufigkeit an den Enden des T1-Chromosoms

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Summary

In phage Tl 35 amber- (am-) mutants were isolated and arranged in 19 cistrons by spot test. The am-mutants (one per cistron) together with some plaque-type mutants were mapped by means of two and three factor crosses. The mutants can be arranged on a linear map, circular linkage could not be detected.

  1. (1)

    The distribution of the cistrons on the map is not random, but a clustering of cistrons is found at the middle of the map. Two possibilities could be responsible for this phenomenon.

  2. (a)

    Most of the DNA at the ends of the chromosome does not code for proteins, or for proteins where am-mutants do not occur.

  3. (b)

    Recombinant frequencies may be low in the middle relative to the ends of the chromosome.

  4. (2)

    As a measurement for recombination at a given point the HET frequency at that point was used. In two factor crosses between am-mutants as well as between plaque-type mutants the fractions of HETs were tested. Markers at the ends were found to be more often heterozygote than markers from the middle of the chromosome. This data seems to support the possibility (b).

  5. (3)

    Marker rescue after high doses of X-rays might also reflect recombination at a given point. Marker rescue frequencies, now, do show a similar distribution as the HET frequencies when plotted versus the map of Tl.

From these three observations it was concluded, that recombination frequency increases sharply towards the ends of the Tl chromosome.

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  1. Institut für Genetik der Universität zu Köln, Koln, Germany

    W. Michalke

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Michalke, W. Erhöhte Rekombinationshäufigkeit an den Enden des T1-Chromosoms. Molec. Gen. Genetics 99, 12–33 (1967). https://doi.org/10.1007/BF00306454

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