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Aprotinin, a carbohydrate-binding protein

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Summary

Evidence is presented for a carbohydrate-binding property of aprotinin, which is preserved both in a fluorescein isothiocyanate (FITC) conjugate and a cyanogen bromidelinked Sepharose conjugate of the protein. Both conjugates similarly retain their tryptic and chymotryptic inhibitory properties. The FITC conjugate is shown to be a single species with respect to charge and to molecular weight and shows a specific binding of normal materials containing sialosyl or uronosyl groups, which accords with its histochemical behaviour. The Sepharose-conjugate showed a similar specificity.

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References

  • Kiernan, J. A., Stoddart, R. W.: Fluorescent-labelled aprotinin: A new reagent for the histochemical detection of acid mucosubstances. Histochemie 34, 77–84 (1973).

    Google Scholar 

  • Neuberger, A., Marshall, R. D.: In: Glycoproteins, their composition, structure and function. B.B.A. Library, vol. 5., chap. 8, Methods for the qualitative and quantitative analysis of the component sugars, p. 190–268, ed. A. Gottschalk, Amsterdam-London-New York: Elsevier Publ. Co. 1966.

    Google Scholar 

  • Stoddart, R. W., Barrett, A. J., Northcote, D. H.: Pectic polysaccharides of growing plant tissues. Biochem. J. 102, 194–204 (1967).

    Google Scholar 

  • Stoddart, R. W., Kiernan, J. A.: Histochemical detection of the α-D-arabinopyranoside configuration using fluorescent labelled concanavalin A. Histochemie 33, 87–94 (1973).

    Google Scholar 

  • Stoddart, R. W., Spires, I. P. C., Tipton, K. F.: Solution properties of polygalacturonic acid. Biochem. J. 114, 863–870 (1969).

    Google Scholar 

  • Vogel, R., Trautsehold, I., Werle, E.: In: Natural proteinase inhibitors, p. 85–93. London-New York: Acad. Press 1968.

    Google Scholar 

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Author notes

  1. J. A. Kiernan

    Present address: Department of Anatomy, Health Sciences Centre, University of Western Ontario, London 72, Ontario, Canada

Authors and Affiliations

  1. Strangeways Research Laboratory, Wort's Causeway, Cambridge, UK

    R. W. Stoddart

  2. Dept. of Anatomy, University of Cambridge, Tennis Court Road, Cambridge, UK

    J. A. Kiernan

Authors
  1. R. W. Stoddart
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  2. J. A. Kiernan
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Additional information

R.W.S. holds a grant from the Cancer Research Campaign. We thank Dr. G. M. W. Cook for discussion and advice.

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Stoddart, R.W., Kiernan, J.A. Aprotinin, a carbohydrate-binding protein. Histochemie 34, 275–280 (1973). https://doi.org/10.1007/BF00306299

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  • DOI: https://doi.org/10.1007/BF00306299

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