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Über die β-Glucosidase und Lactase im Darm von Vertebraten

β-Glucosidase and lactase in the intestine of vertebrates

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Summary

By means of 1-naphthyl derivates β-glucosidase has been demonstrated in the intestine of rats, toads and gold-fishes; lactase can only be detected in rats and toads. The intestine of pigeons, finches, turtles and frogs does not react. With the exception of the toad where both enzymes are localized in the cytoplasm the brush border of the enterocytes is always stained.

In the intestine of suckling rats the highest β-glucosidase and lactase activity occurs dnring the first week of life. At birth these enzymes have already reached their maximal activity. In comparison with the corresponding galactosid 1-naphthyl-β-glucoside exhibits an about five times higher splitting rate. The results obtained in the inhibition tests are nearly the same for β-glucosidase and lactase.

Zusammenfassung

Mit 1-Naphthylderivaten kann die β-Glucosidase im Darm von Ratte, Kröte und Goldfisch nachgewiesen werden; die Lactase kommt nur bei Ratte und Kröte vor. Das Intestinum von Taube, Grünfink, Schildkröte und Frosch reagiert negativ. Mit Ausnahme der Kröte, bei der beide Enzyme im Cytoplasma lokalisiert sind, färbt sich stets der Bürstensaum der Enterozyten an.

Im Darm von Rattensäuglingen besitzen β-Glucosidase und Lactase die höchste Aktivität während der ersten Lebenswoche, wobei das Aktivitätsmaximum bereits am Geburtstermin auftritt. Verglichen mit dem entsprechenden Galactosid wird 1-Naphthyl-β-glucosid etwa 5mal schneller gespalten. Hemmversuche erbringen für die β-Glucosidase und Lactase nahezu identische Resultate.

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Literatur

  • Alvarez, A., Sas, J.: β-galactosidase changes in the developing intestinal tract of the rat. Nature (Lond.) 190, 826–827 (1961).

    Google Scholar 

  • Asp, N.-G., Dahlquist, A.: Assay of 2-naphthyl-β-galactosidase activity. Analyt. Biochem. 42, 275–280 (1971).

    Google Scholar 

  • Barrett, A. J.: Prpoerties of lysosomal enzymes. In: Lysosomes (Dingle, J. T., Fell, H. B., eds.), vol. I, p. 245–312. Amsterdam-London: North-Holland Publishing Co. 1969.

    Google Scholar 

  • Beck, C., Tappel, A. L.: Rat liver lysosomal β-glucosidase: a membrane enzyme. Biochim. biophys. Acta (Amst.) 151, 159–164 (1968).

    Google Scholar 

  • Crane, R. K.: Hypothesis for mechanism of intestinal active transport of sugars. Fed. Proc. 21, 891–895 (1962).

    Google Scholar 

  • Crane, R. K.: A concept of the digestive-absorptive surface of the small intestine. In: Handbook of physiology (C. F. Code, (ed.), Alimentary canal, p. 2535–2542. Washington, D. C.: Amer. Physiol. Soc. 1968.

    Google Scholar 

  • Doell, R. G., Kretschmer, N.: Studies of small intestine during development. I. Distribution and activity of β-galactosidase. Biochim. biophys. Acta (Amst.) 62, 353–362 (1962).

    Google Scholar 

  • Gossrau, R.: Verwendung der Gefriertrocknung nach Lowry in der Histochemie. Histochemie 29, 185–188 (1972a).

    Google Scholar 

  • Gossrau, R.: Über den histnechemischen Nachweis der β-Glucosidase mit l-Naphthyl-β-glucopyranosid. Histochemie (1973a, im Druck).

  • Gossrau, R.: Über den the histochemical demonstration of N-acetyl-β-galactosaminidase. Histochemie 29, 315–324 (1972b).

    Google Scholar 

  • Gossrau, H.: Über den histoochemischen und mikrochemischen Nach weis der β-Galactosidase mit 1-Naphthyl-β-galactopyranosid. Histochemie (1973b, im Druck).

  • Jos, J., Frezal, J., Rey, J., Lamy, M., Wegmann, R.: La localisation histochimique des disaccharidases intestinales avec un procédé nouveau. Ann. Histochim. 12, 53–61 (1967).

    Google Scholar 

  • Kerry, K. R.: Intestinal disaccharidase activity in a montreme and eight species of marsupials (with an added note on the disaccharidases of five species of sea birds). Comp. Biochem. Physiol. 29, 1015–1022 (1969).

    Google Scholar 

  • Koldovsky, O., Asp, N.-G., Dahlquist, O.: A method for the separate assay of “neutral” and “acid” β-galactosidase in homogenates of rat small-intestinal mucosa. Analyt. Biochem. 27, 409–418 (1969).

    Google Scholar 

  • Koldovsky, O., Chytil, F.: Postnatal development of β-galactosidase activity in the small intestine of the rat. (Effect of diet and adrenalectomy). Biochem. J. 94, 266–270 (1965).

    Google Scholar 

  • Kramer, K.: Resorption im Verdauungskanal. In: Physiologie des Menschen (Gauer, O. H., Kramer, K., Jung, R., (Hrsg.), Bd.8, S. 191–195. München-Berlin-Wien: Urban & Schwarzenberg 1972.

    Google Scholar 

  • Lojda, Z.: Some remarks concerning the histochemical detection of disaccharidases and glucosidases. Histochemie 5, 339–360 (1965).

    Google Scholar 

  • Lojda, Z.: Indigogenic methods for glycosidases. I. An improved method for β-glucosidase and its application to localization studies of intestinal and renal enzymes. Histochemie 22, 347–361 (1970a).

    Google Scholar 

  • Lojda, Z.: Indigogenic methods for glycosidases. II. An improved method for β-D-galactosidase and its application to localization studies of the enzymes in the intestine and other tissues. Histochemie 23, 266–288 (1970b).

    Google Scholar 

  • Lojda, Z.: pers. Mitt. (1972).

  • Lojda, Z., Kraml, J.: Indigogenic methods for glycosidases. III. An improved method with 4-Cl-5-Br-3-indolyl-β,-D-fucoside and its application in studies of enzymes in the intestine, kidney and other tissues. Histochemie 25, 195–207 (1971).

    Google Scholar 

  • Malathi, P., Crane, R. K.: β-glucosidase (phlorizin hydrolase) activity in intestinal brush border. Fed. Proc. 27, 385 (1968).

    Google Scholar 

  • Malathi, P., Crane, R. K.: Phlorizin hydrolase: a β-glucosidase of hamster intestinal brush border membrane. Biochim. biophys. Acta (Amst.) 178, 245–256 (1969).

    Google Scholar 

  • Nordström, Ch., Koldovsky, O., Dahlquist, A.: Localization of β-galactosidases and acid phosphatase in the small intestinal wall. Comparison of adult and suckling rat. J. Histochem. Cytochem. 17, 341–347 (1969).

    Google Scholar 

  • Parsons, D. S., Prichard, J. S.: Hydrolysis of disaccharides during absorption by the perfused small intestine of amphibia. Nature (Lond.) 208, 1097–1098 (1965).

    Google Scholar 

  • Rubino, A., Zimbalatti, F., Auricchio, S.: Intestinal disaccharidase activities in adult and suckling rats. Biochim. biophys. Acta (Amst.) 92, 305–311 (1964).

    Google Scholar 

  • Semenza, G.: Intestinal oligosaccharidases and disaccharidases. In: Handbook of physiology (C.F. Code, ed.), Alimentary canal, p. 2547–2566. Washington, D. C.: Amer. Physiol. Soc. 1968.

    Google Scholar 

  • Semenza, G., Auricchio, S., Rubino, A.: Multiplicity of human intestinal disaccharidases. I. Chromatographic separation of maltases and two lactases. Biochim. biophys. Acta (Amst.) 96, 487–497 (1965).

    Google Scholar 

  • Veibel, S.: β-Glucosidase. In: The enzymes (Summer, J. B., Myrbäck, K., eds.), vol. I, part I, p. 583–620. New York: Academic Press 1950.

    Google Scholar 

  • Vollrath, L.: Über die Entwicklung des Dünndarms der Ratte. Morphologische, histochemische und experimentelle Untersuchungen. Ergebn. Anat. Entwickl.-Gesch. 41, 7–70 (1969).

    Google Scholar 

  • Weinreb, N. J., Brandy, R. O., Tappel, A. L.: The lysosomal localization of sphingolipid hydrolases. Biochim. biophys. Acta (Amst.) 159, 141–146 (1968).

    Google Scholar 

  • Winckler, J.: Zum Einfrieren von Gewebe in Stickstoff-gekühltem Propan. Histochemie 23, 44–50 (1970a).

    Google Scholar 

  • Winckler, J.: Verwendung gefriergetrockneter Kryostatschnitte für histologische und histochemische Untersuchungen. Histochemie 24, 168–186 (1970b).

    Google Scholar 

  • Zoppi, G., Shmerling, D. H.: Intestinal disaccharidase activities in some birds, reptiles and mammals. Comp. Biochem. Physiol. 29, 289–294 (1969).

    Google Scholar 

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  1. Anatomisches Institut der Universität Würzburg, Deutschland

    R. Gossrau

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Gossrau, R. Über die β-Glucosidase und Lactase im Darm von Vertebraten. Histochemie 35, 143–151 (1973). https://doi.org/10.1007/BF00306040

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