Summary
To clarify the yet controversial fine structure of Hirano bodies, we made three-dimensional observations of the tissues from the right hippocampus obtained at autopsy of elderly patients by the quick-freeze, deep-etch and replica method. The basic structure of Hirano bodies was a unit lamella, a closely attached pair of sheets composed of parallel-running smooth filaments, 10 to 12 nm in diameter with 12-nm interspaces. In the unit lamella, filaments from each of the overlapping sheets crossed obliquely at acute or obtuse angles to form lattice-like meshworks. The unit lamellae were arranged in a folded, waved or concentric manner, and connected or supported by cross-linking filaments of the same width. The distance between these unit lamellae was about 50 nm. Occasionally the sheets were separated or fused making layers of one to three sheets. At the periphery of the bodies parallel filaments were dispersed into individual filaments of similar size or directly attached to the cytoplasmic membrane.
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Cavanagh JB, Blakemore WF, Kyu MH (1971) Fibrillary accumulation in oligodendroglial processes of rats subjected to portocaval anastomosis. J Neurol Sci 14: 143–152
Field EJ, Narang HK (1972) An electron microscopic study of scrapie in the rat: further observations on “inclusion bodies” and virus-like particles. J Neurol Sci 17: 347–364
Galloway PG, Perry G, Gambetti P (1987) Hirano body filaments contain actin and actin-associated proteins. J Neuropathol Exp Neurol 46: 185–199
Galloway PG, Perry G, Kosik KS, Gambetti P (1987) Hirano bodies contain tau protein. Brain Res 403: 337–340
Gabson PH (1978) Light and electron microscopic obserations on the relationship between Hirano bodies, neuron and glial perikarya in the human hippocampus. Acta Neuropathol (Berl) 42: 165–171
Gibson PH, Tomlinson BE (1977) Numbers of Hirano bodies in the hippocampus of normal and demented people with Alzheimer's disease. J Neurol Sci 33: 199–206
Goldman JE (1983) The association of actin with Hirano bodies. J Neuropathol Exp Neurol 42: 146–152
Heuser JE, Kirschner MW (1980) Filament organization revealed in platinum replicas of freeze-dried cytoskeletons. J Cell Biol 86: 212–234
Hirano A (1965) Pathology of amyotrophic lateral sclerosis. In: Gajdusek DC, Gibbs CJ Jr, Aopers M (eds) Slow, latent and temperate virus infections. NINDB Monograph No 2. National Institute of Health, Washington D.C., pp 23–27
Hirano A (1978) A possible mechanism of demyelination in the Syrian hamster with hindleg paralysis. Lab Invest 38: 115–121
Hirano A, Dembitzer HM (1976) Eosinophilic rod-like structures in myelinated fibers of hamster spinal root. Neuropathol Appl Neurobiol 2: 225–232
Hirano A, Dembitzer HM, Kurland LT, Zimmerman HM (1968) The fine structure of some intraganglionic alterations. Neurofibrillary tangles, granulovacuolar bodies and “rod-like” structures as seen in Guam amyotrophic lateral sclerosis and parkinsonism-dementia complex. J Neuropathol Exp Neurol 27: 167–182
Hirano A, Dembitzer HM, Zimmerman HM (1973) The fine structure of phosphotungstic acid stained neuropathologic tissue. Acta Neuropathol (Berl) 26: 265–272
Mori H, Tomonaga M, Baba N, Kanaya K (1986) The structure analysis of Hirano bodies by digital processing on electron micrographs. Acta Neuropathol (Berl) 71: 32–37
O'Brien L, Shelley K, Towfighi J, McPherson A (1980) Crystalline ribosomes are present in brains from senile humans. Proc Natl Acad Sci USA 77: 2260–2264
Ogata J, Budzilovich GN, Cravioto H (1972) A study of rodlike structures (Hirano bodies) in 240 normal and pathological brains. Acta Neuropathol (Berl) 21: 61–67
Ohtsubo K, Izumiyama N, Shimada H, Tachikawa T, Nakamura H (1990) Three-dimensional structure of Alzheimer's neurofibrillary tangles of the aged human brain revealed by quick-freeze, deep-etch and replica method. Acta Neuropathol 79: 480–485
Ohtsubo K, Izumiyama N, Kuzuhara S, Mori H, Shimada H (1990) Curly fibers are tau-positive strands in the pre- and post-synaptic neurites, consisting of paired helical filaments —Observations by freeze-etch and replica method. Acta Neuropathol (in press)
Peterson C, Kress Y, Vallee R, Goldman JE (1988) High molecular weight microtubule-associated proteins bind to actin lattices (Hirano bodies). Acta Neuropathol 77: 168–174
Schmidt ML, Lee VM-Y, Trojanowski JQ (1989) Analysis of epitopes shared by Hirano bodies and neurofilament proteins in normal and Alzheimer's disease hippocampus. Lab Invest 60: 513–522
Schochet SS Jr, McCormick WF (1972) Ultrastructure of Hirano bodies. Acta Neuropathol (Berl) 21: 50–60
Tomonaga M (1974) Ultrastructure of Hirano bodies. Acta Neuropathol (Berl) 28: 365–366
Tomonaga M (1983) Hirano body in extraocular muscle. Acta Neuropathol (Berl) 60: 309–313
Yagishita S, Itoh Y, Nakano T, Ono Y, Amano N (1979) Crystalloid inclusions reminiscent of Hirano bodies in autolyzed peripheral nerve of normal Wistar rats. Acta Neuropathol (Berl) 47: 213–236
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Izumiyama, N., Ohtsubo, K., Tachikawa, T. et al. Elucidation of three-dimensional ultrastructure of Hirano bodies by the quick-freeze, deep-etch and replica method. Acta Neuropathol 81, 248–254 (1991). https://doi.org/10.1007/BF00305865
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DOI: https://doi.org/10.1007/BF00305865