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Localization of iodopsin and rod-opsin immunoreactivity in the retina and pineal complex of the river lamprey, Lampetra japonica

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Abstract

The aim of this study has been to examine whether iodopsin immunoreaction exists in the photoreceptor cells of the retina of the river lamprey, Lampetra japonica, and whether this immunoreaction also appears in the photoreceptors of the pineal complex. The lamprey retina possesses long and short photoreceptor cells that display iodopsin immunoreactivity and rod-opsin immunoreactivity, respectively. In the pineal organ, iodopsin immunoreaction has been observed in the peripheral region and the dorsal wall of the end-vesicle. Immunoreactivity is also found in the atrium and the pineal stalk. The iodopsin-immunoreactive outer segments are smaller than those displaying rod-opsin immunoreactivity. In the parapineal organ, iodopsin immunoreactivity is distributed in both dorsal and ventral portions. Double immunostaining has been employed to investigate whether iodopsin and serotonin immunoreactivities are colocalized in one and the same cell. This approach has revealed that the iodopsin-immunoreactive outer segments belong to serotonin-immunopositive and to serotonin-immunonegative photoreceptor cells. These results demonstrate that rod-type or cone-type visual pigments are contained in both typical and modified pineal photoreceptors.

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Authors and Affiliations

  1. First Department of Physiology, Hamamatsu University, School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan

    S. Tamotsu & Y. Morita

  2. Department of Biology, Faculty of Science, Nara Women's University, Nara, Japan

    T. Oishi & K. Nakao

  3. Department of Biophysics, Faculty of Science, Kyoto University, Kyoto, Japan

    Y. Fukada & Y. Shichida

  4. Department of Applied Physics and Chemistry, The University of Electro-Communications, Chofu, Japan

    T. Yoshizawa

Authors
  1. S. Tamotsu
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  2. T. Oishi
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  3. K. Nakao
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  4. Y. Fukada
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  5. Y. Shichida
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  6. T. Yoshizawa
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  7. Y. Morita
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Tamotsu, S., Oishi, T., Nakao, K. et al. Localization of iodopsin and rod-opsin immunoreactivity in the retina and pineal complex of the river lamprey, Lampetra japonica . Cell Tissue Res 278, 1–10 (1994). https://doi.org/10.1007/BF00305772

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  • DOI: https://doi.org/10.1007/BF00305772

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