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Functional mitochondria in snake Bothrops alternatus erythrocytes and modulation of HbO2 affinity by mitochondrial ATP

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Abstract

Digitonin was applied to permeabilize the plasma membrane of Bothrops alternatus erythrocytes to study respiration, oxidative phosphorylation and Ca2+ transport by mitochondria in situ. These mitochondria oxidized added NAD-linked substrates, succinate and N,N,N′, N′-tetramethyl-p-phenylenediamine. Respiration was sensitive to rotenone and cyanide but not to antimycin A. This indicates that Bothrops mitochondria possess the respiratory complexes NADH-ubiquinone, succinate-ubiquinone, and ferrocytochrome c-oxygen oxidoreductases, although the lack of sensitivity to antimycin A raises doubt about the composition of the ubiquinol cytochrome c-reductase complex. An ability to build up and sustain a membrane potential was documented by their capacity to accumulate tetraphenylphosphonium and Ca2+ through an uncoupler-sensitive mechanism. Addition of ADP caused a transient decrease in the membrane potential, indicating that this is the predominant driving force for ATP synthesis as in most types of mitochondria. Uncoupling of phosphorylation from the oxidative process increased hemoglobin O2 affinity, which suggests that ATP production by mitochondria may participate in modulation of O2 transport by hemoglobin.

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Abbreviations

ΔΨ:

membrane potential

BAE:

Bothrops alternatus erythrocytes

DNP:

2,4-dinitrophenol

DPG:

2,3-diphosphoglycerate

EGTA:

ethyleneglycol tetra-acetic acid

FCCP:

carbonylcyanide p-trifloromethoxyphenylhydrazone

TMPD:

N,N,N′,N′-tetramethyl-p-phenylenediamine

TPP+ :

tetraphenylphosphonium

TRIS:

tris-(hydroxymethyl)aminomethane

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Authors and Affiliations

  1. Departmento de Bioquimica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil

    S. H. Ogo, C. F. Bernardes, M. A. Torsoni & A. E. Vercesi

  2. Departamento de Bioquimica, Pontifica Universidade Catolica de Campinas, Campinas, SP, Brazil

    C. F. Bernardes

  3. Departamento de Fisiologia, Faculdade de Medicina de Ribeirao Preto, USP, Ribeirao Preto, SP, Brazil

    M. L. Glass

Authors
  1. S. H. Ogo
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  2. C. F. Bernardes
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  3. M. L. Glass
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  4. M. A. Torsoni
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  5. A. E. Vercesi
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Ogo, S.H., Bernardes, C.F., Glass, M.L. et al. Functional mitochondria in snake Bothrops alternatus erythrocytes and modulation of HbO2 affinity by mitochondrial ATP. J Comp Physiol B 163, 614–619 (1993). https://doi.org/10.1007/BF00302122

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  • DOI: https://doi.org/10.1007/BF00302122

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