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Phylogenic relationships of the amino acid sequences of prosome (proteasome, MCP) subunits

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Abstract

Prosomes [or proteasomes, Multi-Catalytic Proteinase (MCP)] are multisubunit protein complexes, found from archaebacteria to man, the structure of which (a 4-layer cylinder) is remarkably conserved. They were first observed as subcomplexes of untranslated mRNP, and then as a multicatalytic proteinase with several proteolytic activities. A number of sequences from subunits of these complexes are now available. Analysis of the sequences shows that these subunits are evolutionarily related, and reveals three highly conserved amino acid stretches. Based on a phylogenic approach, we propose to classify the sequenced subunits into 14 families, which fall into two superfamilies, of the α- and β-type. These data, together with several recently published observations, suggest that some subunits may be interchangeable within the complexes, which would thus constitute a population of heterogenous particles.

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  1. O. Coux

    Present address: Department of Cell Biology, Harvard Medical School, 25 Shattuck Street, 02115, Boston, MA, USA

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  1. Institut Jacques Monod CNRS, Université Paris 7, 2 place Jussieu, F-75251, Paris Cedex 05, France

    O. Coux, H. G. Nothwang, I. Silva Pereira, F. Recillas Targa, F. Bey & K. Scherrer

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Coux, O., Nothwang, H.G., Silva Pereira, I. et al. Phylogenic relationships of the amino acid sequences of prosome (proteasome, MCP) subunits. Molec. Gen. Genet. 245, 769–780 (1994). https://doi.org/10.1007/BF00297284

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