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Dynamics of actin filaments in microglia during Fc receptor-mediated phagocytosis

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Abstract

The phagocytic ability of mouse microglia during their differentiation in culture and after stimulation with bacterial wall lipopolysaccharide (LPS) has been investigated using Fc receptor-mediated phagocytosis of immunoglobulin (IgG)-coated sheep erythrocytes (SRBCs). We observed that in 10–14 day-confluent neopallial cell cultures some immature microglia are not phagocytic. LPS-stimulated microglia are able to phagocytose larger numbers of IgG-coated SRBCs and at a faster rate than non-stimulated microglia. Within 5–10 min of phagocytosis the actin filaments of the LPS-stimulated microglia become depolymerized, leaving only bundles of actin filaments around the phagocytosed SRBCs (phagosome cups). At 30 min after the start of phagocytosis the actin filaments of the LPS-stimulated microglia begin to polymerize, and within 2 h the original pre-phagocytosis pattern of the actin filament network is re-established. The non-LPS-stimulated microglia exhibit actin filament depolymerization in only a few lamellipodia and polymerization of actin filaments around engulfed particles, but much later during phagocytosis.

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Authors and Affiliations

  1. Network of Centre of Excellence for Neural Regeneration and Network of Centre of National Recovery Department of Anatomy, College of Medicine, University of Saskatchewan, S7N OWO, Saskatoon, SK, Canada

    E. M. Abd-El-Basset & S. Fedoroff

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  1. E. M. Abd-El-Basset
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  2. S. Fedoroff
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Supported by Medical Research Council of Canada Grant MT4235 to S. F. and a Centres of Excellence fellowship to E. M.

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Abd-El-Basset, E.M., Fedoroff, S. Dynamics of actin filaments in microglia during Fc receptor-mediated phagocytosis. Acta Neuropathol 88, 527–537 (1994). https://doi.org/10.1007/BF00296489

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  • DOI: https://doi.org/10.1007/BF00296489

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